Silk fibroin/poly(L‑lactic acid‑co‑ε‑caprolactone) electrospun nanofibrous scaffolds exert a protective effect following myocardial infarction

Du,Mingjun; Gu,Jianmin; Wang,Juan; Xue,Yizheng; Ma,Yiwen; Mo,Xiumei; Xue,Song

doi:10.3892/etm.2019.7405

Silk fibroin/poly(L‑lactic acid‑co‑ε‑caprolactone) electrospun nanofibrous scaffolds exert a protective effect following myocardial infarction

Authors:
- Mingjun Du
- Jianmin Gu
- Juan Wang
- Yizheng Xue
- Yiwen Ma
- Xiumei Mo
- Song Xue
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Affiliations: Department of Cardiovascular Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P.R. China, College of Clinical Medicine, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, P.R. China, Department of Anesthesiology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, P.R. China
Published online on: March 18, 2019 https://doi.org/10.3892/etm.2019.7405
Pages: 3989-3998
Copyright: © Du et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Electrospinning using biocompatible polymer scaffolds, seeded with or without stem cells, is considered a promising technique for producing fibrous scaffolds with therapeutic possibilities for ischemic heart disease. However, no optimal scaffolds for treating ischemic heart disease have been identified thus far. In the present study, it was evaluated whether electrospun silk fibroin (SF)‑blended poly(L‑lactic acid‑co‑ε‑caprolactone) [P(LLA‑CL)] scaffolds that were seeded with cluster of differentiation 117 (c‑kit)+ bone marrow (BM) cells may serve a protective role in cardiac remodeling following myocardial infarction (MI). Mechanical characteristics and cytocompatibility were compared between SF/P(LLA‑CL) and P(LLA‑CL) electrospun nanofibrous scaffolds in vitro. It was observed that MI led to a significant increase of the c‑kit+ BM cell subpopulation in mice. Magnetic activated cell sorting was performed to harvest the c‑kit+ cell population from the BM of mice following MI. c‑kit+ BM cells were seeded on SF/P(LLA‑CL) and P(LLA‑CL) electrospun nanofibrous scaffolds. Results indicated that SF/P(LLA‑CL) electrospun nanofibrous scaffolds were superior to P(LLA‑CL) electrospun nanofibrous scaffolds in improving c‑kit+ BM cell proliferation. Additionally, compared with pure SF/P(LLA‑CL) electrospun nanofibrous scaffolds, SF/P(LLA‑CL) scaffolds seeded with c‑kit+ BM cells resulted in lower levels of MI markers and reduced infarct size, leading to greater global heart function improvement in vivo. The findings of the present study indicated that SF/P(LLA‑CL) electrospun nanofibrous scaffolds seeded with c‑kit+ BM cells exert a protective effect against MI and may be a promising approach for cardiac regeneration after ischemic heart disease.

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