Downregulation of microRNA‑425‑5p suppresses cervical cancer tumorigenesis by targeting AIFM1

Zhang,Ying; Yang,Yuxiu; Liu,Rongxia; Meng,Yucui; Tian,Geng; Cao,Qinying

doi:10.3892/etm.2019.7408

Downregulation of microRNA‑425‑5p suppresses cervical cancer tumorigenesis by targeting AIFM1

Authors:
- Ying Zhang
- Yuxiu Yang
- Rongxia Liu
- Yucui Meng
- Geng Tian
- Qinying Cao
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Affiliations: Department of Gynecology and Obstetrics, Shijiazhuang Obstetrics and Gynecology Hospital, Shijiazhuang, Hebei 050011, P.R. China, Department of Epidemiology, Shijiazhuang Obstetrics and Gynecology Hospital, Shijiazhuang, Hebei 050011, P.R. China, Department of Reproductive Medicine, Shijiazhuang Obstetrics and Gynecology Hospital, Shijiazhuang, Hebei 050011, P.R. China
Published online on: March 18, 2019 https://doi.org/10.3892/etm.2019.7408
Pages: 4032-4038
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Although microRNA‑425‑5p (miR‑425‑5p) has been previously revealed to be upregulated in cervical cancer, the cellular function of miR‑425‑5p in cervical cancer remains unknown. The aim of the current study was to investigate the cellular function of miR‑425‑5p and its underlying mechanism in cervical cancer. Reverse transcription‑quantitative polymerase chain reaction was used to measure miR‑425‑5p expression in several cervical cancer cell lines. TargetScan bioinformatics analysis was used to predict apoptosis‑inducing factor mitochondria‑associated 1 (AIFM1) as a novel target of miR‑425‑5p, and this was verified by dual‑luciferase reporter assay. Furthermore, cell transfections were used to investigate the role of miR‑425‑5p in cervical cancer. The effect of miR‑425‑5p on cell viability and apoptosis in HeLa cells was detected by MTT assay and flow cytometry, respectively. The present study demonstrated that miR‑425‑5p was significantly upregulated in cervical cancer cell lines. In addition, AIFM1 was identified as a direct target of miR‑425‑5p and negatively regulated by miR‑425‑5p. Downregulation of miR‑425‑5p inhibited HeLa cell viability and induced cell apoptosis. Furthermore, downregulation of miR‑425‑5p significantly increased the protein and mRNA expression levels of cytochrome c, caspase‑3, caspase‑9 and DNA damage regulated autophagy modulator 1. The effects of miR‑425‑5p inhibition on HeLa cell viability and apoptosis were significantly reversed by AIFM1 knockdown. In conclusion, the present study demonstrated that miR‑425‑5p was upregulated in cervical cancer, and downregulation of miR‑425‑5p inhibited cervical cancer cell growth by targeting AIFM1.

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