Open Access

Identification of key candidate genes and pathways in oral squamous cell carcinoma by integrated Bioinformatics analysis

  • Authors:
    • Bo Zou
    • Jun Li
    • Kai Xu
    • Jian‑Lin Liu
    • Dao‑Ying Yuan
    • Zhen Meng
    • Bin Zhang
  • View Affiliations

  • Published online on: March 26, 2019     https://doi.org/10.3892/etm.2019.7442
  • Pages: 4089-4099
  • Copyright: © Zou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Oral squamous cell carcinoma (OSCC) is one of the most common types of malignant head and neck tumor, which poses a serious threat to human health. In recent years, the incidence of OSCC has been increasing, while the prognosis has not significantly improved. Elucidation of the molecular mechanisms underlying the development of OSCC may provide novel therapeutic strategies. In the present study, the gene expression profiles from 4 datasets, including 244 OSCC and 95 normal oral mucosa samples, were subjected to statistical and Bioinformatics analysis. A total of 34 differentially expressed genes (DEGs) were identified, among which 14 were upregulated and 20 were downregulated in OSCC compared with normal oral mucosa tissues. Gene Ontology enrichment analysis indicated that the DEGs were mainly involved in regulation of the immune response, cell adhesion and cell proliferative processes. The Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that the DEGs were mainly associated with the phosphoinositide‑3 kinase Akt and Toll‑like receptor signaling pathway. The key candidate DEGs were identified from the complex protein‑protein interaction network, and secreted phosphoprotein 1 (SPP1), integrin subunit α 3 and plasminogen activator, urokinase (PLAU) were confirmed to be significantly associated with the survival rate. Cell Counting Kit‑8 and Transwell assays demonstrated that SPP1 and PLAU regulate cell proliferation, migration and invasion. The candidate genes/pathways identified in the present study may include promising diagnostic biomarkers or therapeutic targets for OSCC.
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May-2019
Volume 17 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zou B, Li J, Xu K, Liu JL, Yuan DY, Meng Z and Zhang B: Identification of key candidate genes and pathways in oral squamous cell carcinoma by integrated Bioinformatics analysis. Exp Ther Med 17: 4089-4099, 2019.
APA
Zou, B., Li, J., Xu, K., Liu, J., Yuan, D., Meng, Z., & Zhang, B. (2019). Identification of key candidate genes and pathways in oral squamous cell carcinoma by integrated Bioinformatics analysis. Experimental and Therapeutic Medicine, 17, 4089-4099. https://doi.org/10.3892/etm.2019.7442
MLA
Zou, B., Li, J., Xu, K., Liu, J., Yuan, D., Meng, Z., Zhang, B."Identification of key candidate genes and pathways in oral squamous cell carcinoma by integrated Bioinformatics analysis". Experimental and Therapeutic Medicine 17.5 (2019): 4089-4099.
Chicago
Zou, B., Li, J., Xu, K., Liu, J., Yuan, D., Meng, Z., Zhang, B."Identification of key candidate genes and pathways in oral squamous cell carcinoma by integrated Bioinformatics analysis". Experimental and Therapeutic Medicine 17, no. 5 (2019): 4089-4099. https://doi.org/10.3892/etm.2019.7442