Open Access

Methylation‑regulated ZNF545 inhibits growth of the p53‑mutant KYSE150 cell line by inducing p21 and Bax

Corrigendum in: /10.3892/etm.2021.9948

  • Authors:
    • Yu Fan
    • Yu Wang
    • Shaozhi Fu
    • Duan Liu
    • Sheng Lin
  • View Affiliations

  • Published online on: July 5, 2019     https://doi.org/10.3892/etm.2019.7737
  • Pages: 1563-1570
  • Copyright: © Fan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The Krüppel‑associated box zinc‑finger protein 545 (ZNF545) was recently identified as a tumor suppressor in esophageal squamous cell carcinoma (ESCC). However, the role of ZNF545 in the tumorigenesis of esophageal cancer cells expressing loss‑of‑function mutant p53 has not been elucidated. In the present study, the role of ZNF545 in esophageal tumors and the p53‑mutant ESCC cell line, KYSE150, was investigated. ZNF545 mRNA was significantly downregulated in tumors when compared with adjacent normal tissues. Methylated ZNF545 was detected in 76.6% of tumor tissues compared with 28.1% of adjacent normal tissues. Combined pharmacological treatment of KYSE150 cells with a demethylating reagent and deacetylase inhibitor restored the expression of ZNF545. Ectopic expression of ZNF545 activated p53 transcription and upregulated the protein expression levels of pivotal effectors p21 and Bax, which are associated with cell proliferation and apoptosis, respectively, in p53‑mutant KYSE150 cells; while suppressing colony formation and inducing apoptosis. ZNF545 was therefore proposed as a potential tumor suppressor responsible for inhibiting the growth of p53‑mutant ESCC cell lines. In addition, tumor‑specific methylation of ZNF545 may represent an epigenetic diagnostic biomarker and a therapeutic target in patients with esophageal cancer.
View Figures
View References

Related Articles

Journal Cover

September-2019
Volume 18 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Fan Y, Wang Y, Fu S, Liu D and Lin S: Methylation‑regulated ZNF545 inhibits growth of the p53‑mutant KYSE150 cell line by inducing p21 and Bax Corrigendum in /10.3892/etm.2021.9948. Exp Ther Med 18: 1563-1570, 2019.
APA
Fan, Y., Wang, Y., Fu, S., Liu, D., & Lin, S. (2019). Methylation‑regulated ZNF545 inhibits growth of the p53‑mutant KYSE150 cell line by inducing p21 and Bax Corrigendum in /10.3892/etm.2021.9948. Experimental and Therapeutic Medicine, 18, 1563-1570. https://doi.org/10.3892/etm.2019.7737
MLA
Fan, Y., Wang, Y., Fu, S., Liu, D., Lin, S."Methylation‑regulated ZNF545 inhibits growth of the p53‑mutant KYSE150 cell line by inducing p21 and Bax Corrigendum in /10.3892/etm.2021.9948". Experimental and Therapeutic Medicine 18.3 (2019): 1563-1570.
Chicago
Fan, Y., Wang, Y., Fu, S., Liu, D., Lin, S."Methylation‑regulated ZNF545 inhibits growth of the p53‑mutant KYSE150 cell line by inducing p21 and Bax Corrigendum in /10.3892/etm.2021.9948". Experimental and Therapeutic Medicine 18, no. 3 (2019): 1563-1570. https://doi.org/10.3892/etm.2019.7737