Bone morphogenetic protein‑7 upregulates genes associated with osteoblast differentiation, including collagen I, Sp7 and IBSP in gingiva‑derived stem cells

Lee,Hyunjin; Min,Sae Kyung; Song,Youngmin; Park,Yun‑Hee; Park,Jun‑Beom

doi:10.3892/etm.2019.7904

Bone morphogenetic protein‑7 upregulates genes associated with osteoblast differentiation, including collagen I, Sp7 and IBSP in gingiva‑derived stem cells

Authors:
- Hyunjin Lee
- Sae Kyung Min
- Youngmin Song
- Yun‑Hee Park
- Jun‑Beom Park
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Affiliations: Department of Periodontics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea, Εbiogen Inc., Seoul 07282, Republic of Korea
Published online on: August 16, 2019 https://doi.org/10.3892/etm.2019.7904
Pages: 2867-2876
Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study was performed to evaluate the effects of short‑term application of bone morphogenetic protein‑7 (BMP‑7) on human gingiva‑derived mesenchymal stem cells with next‑generation sequencing. Human gingiva‑derived stem cells were treated with a final concentration of 100 ng/ml BMP‑7 and the same concentration of a vehicle control. mRNA sequencing and data analysis were performed along using gene ontology and pathway analysis. RT‑qPCR of mRNA of collagen I, Sp7, IBSP and western blot analysis of collagen I, osterix and bone sialoprotein was also performed. A total of 25,737 mRNAs were identified to be differentially expressed. Regarding osteoblast differentiation, 14 mRNAs were upregulated and 10 were downregulated when the results of the BMP‑7 at 3 h were compared with the control at 3 h. The expression of collagen I was increased following the application of BMP‑7 at 3 h, and this increase was also observed following western blot analysis. The effects of BMP‑7 on stem cells were evaluated with mRNA sequencing, and the expression was validated with RT‑qPCR and western blot analysis. The short‑term application of BMP‑7 produced an increased expression of collagen I, which was associated with target genes selected for osteoblast differentiation. This study may provide novel insights into the role of BMP‑7 using mRNA sequencing.

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