Enhanced mitochondrial biogenesis is associated with the ameliorative action of creatine supplementation in rat soleus and cardiac muscles

Gowayed,Mennatallah  A.; Mahmoud,Shimaa  A.; El‑Sayed,Yousria; Abu‑Samra,Nehal; Kamel,Maher   A.

doi:10.3892/etm.2019.8173

Enhanced mitochondrial biogenesis is associated with the ameliorative action of creatine supplementation in rat soleus and cardiac muscles

Authors:
- Mennatallah A. Gowayed
- Shimaa A. Mahmoud
- Yousria El‑Sayed
- Nehal Abu‑Samra
- Maher A. Kamel
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Affiliations: Department of Pharmacology and Therapeutics, Faculty of Pharmacy and Drug Manufacturing, Pharos University in Alexandria, Alexandria 21311, Egypt, Department of Biochemistry, Medical Research Institute, Alexandria University, Alexandria 21561, Egypt, Department of Physiology, Medical Research Institute, Alexandria University, Alexandria 21561, Egypt, Department of Basic Sciences, Faculty of Physical Therapy, Pharos University in Alexandria, Alexandria 21311, Egypt, Department of Biochemistry, Medical Research Institute, Alexandria University, Alexandria, Alexandria 21561, Egypt
Published online on: November 7, 2019 https://doi.org/10.3892/etm.2019.8173
Pages: 384-392

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Abstract

The current study focused on the effect of creatine supplementation with/without exercise on the expression of genes controlling mitochondrial biogenesis in skeletal and cardiac muscles, as well as its safety profile on the liver and kidney. A total of 40 male Wister rats were included in the present study. Two unexercised groups: The control sedentary group and the sedentary creatine‑treated group (n=10) were treated daily with oral creatine (0.5 g/kg per day). Two exercised groups performed swimming exercise training 5 days/week for a period of 5 weeks; The Exercise training group, and exercise training and creatine (0.5 g/kg per day) treated group. After sacrifice, blood samples, cardiac and soleus muscles were collected for assessment of mtDNA copy number, gene expression analysis and nuclear extraction for the assay of PGC‑1α. The results of the current study demonstrated that, physical activity with short‑term creatine supplementation increased all factors of mitochondrial biogenesis, an effect that is devoid of any kidney or liver adverse effects. Further studies are still required to explore the potential of creatine supplementation in ameliorating mitochondrial diseases, including epilepsy, skeletal and cardiac myopathies, hepatopathies and nephropathies.

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