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Therapeutic effects of nerve leachate‑treated adipose‑derived mesenchymal stem cells on rat sciatic nerve injury

  • Authors:
    • Yumei Liu
    • Ruiqi Dong
    • Chunyan Zhang
    • Yuxiang Yang
    • Yaolu Xu
    • Haojie Wang
    • Mengyu Zhang
    • Jiamin Zhu
    • Yuqin Wang
    • Yanhong Sun
    • Ziqiang Zhang

  • View Affiliations

  • Published online on: November 15, 2019     https://doi.org/10.3892/etm.2019.8203
  • Pages: 223-231

  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Peripheral nerve injury (PNI) is a common condition, often resulting from physical nerve injury and trauma. Successful repair of the peripheral nerve is dependent on the regenerative activity of Schwann cells (SCs). Application of SC‑like adipose‑derived mesenchymal stem cells (ADSCs) may be a suitable cell‑based therapy for PNI. In the present study, nerve leachate derived from the rat sciatic nerve was used to induce the differentiation of ADSCs. These cells were placed in an acellular biological scaffold, which was then grafted to a rat sciatic nerve to bridge a 1‑cm gap. Sprague‑Dawley rats were divided into four groups: Scaffold only, untreated ADSCs + scaffold, nerve leachate‑treated ADSCs + scaffold and autograft. Two‑months post‑transplant, the structure and function of the regenerated nerves and the recovery of the innervated muscles was analyzed. After transplant, there was a significant increase in the average area (15.86%; P<0.05), density (23.13%; P<0.05) and thickness (43.24%; P<0.05) of regenerated nerve fibers in the nerve leachate‑treated ADSCs + scaffold group compared with the untreated ADSCs + scaffold group. The nerve conduction velocity in the nerve leachate‑treated ADSCs + scaffold and autograft groups was superior to that in the other groups. In the nerve leachate‑treated ADSCs + scaffold group, the cross‑sectional area of the gastrocnemius increased by 39.28% (P<0.05) and the cross‑sectional area of collagen fibers decreased by 29.87% (P<0.05) compared with the ADSCs + scaffold group. Moreover, the therapeutic effect of nerve leachate‑treated ADSCs + scaffold on PNI was similar to that of an autograft. These results suggest that nerve leachate‑treated ADSCs may promote the repair of PNI.
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January-2020
Volume 19 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu Y, Dong R, Zhang C, Yang Y, Xu Y, Wang H, Zhang M, Zhu J, Wang Y, Sun Y, Sun Y, et al: Therapeutic effects of nerve leachate‑treated adipose‑derived mesenchymal stem cells on rat sciatic nerve injury. Exp Ther Med 19: 223-231, 2020.
APA
Liu, Y., Dong, R., Zhang, C., Yang, Y., Xu, Y., Wang, H. ... Zhang, Z. (2020). Therapeutic effects of nerve leachate‑treated adipose‑derived mesenchymal stem cells on rat sciatic nerve injury. Experimental and Therapeutic Medicine, 19, 223-231. https://doi.org/10.3892/etm.2019.8203
MLA
Liu, Y., Dong, R., Zhang, C., Yang, Y., Xu, Y., Wang, H., Zhang, M., Zhu, J., Wang, Y., Sun, Y., Zhang, Z."Therapeutic effects of nerve leachate‑treated adipose‑derived mesenchymal stem cells on rat sciatic nerve injury". Experimental and Therapeutic Medicine 19.1 (2020): 223-231.
Chicago
Liu, Y., Dong, R., Zhang, C., Yang, Y., Xu, Y., Wang, H., Zhang, M., Zhu, J., Wang, Y., Sun, Y., Zhang, Z."Therapeutic effects of nerve leachate‑treated adipose‑derived mesenchymal stem cells on rat sciatic nerve injury". Experimental and Therapeutic Medicine 19, no. 1 (2020): 223-231. https://doi.org/10.3892/etm.2019.8203