lncRNA HOXB‑AS3 protects doxorubicin‑induced cardiotoxicity by targeting miRNA‑875‑3p

Lu,Qun; Huo,Jianhua; Liu,Ping; Bai,Ling; Ma,Aiqun

doi:10.3892/etm.2019.8335

lncRNA HOXB‑AS3 protects doxorubicin‑induced cardiotoxicity by targeting miRNA‑875‑3p

Authors:
- Qun Lu
- Jianhua Huo
- Ping Liu
- Ling Bai
- Aiqun Ma
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Affiliations: Department of Cardiovascular Medicine, First Affiliated Hospital of Medical College and Institute of Cardiovascular Channelopathy, Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), The Ministry of Education, Key Laboratory of Molecular Cardiology, Xi'an, Shaanxi 710061, P.R. China
Published online on: December 16, 2019 https://doi.org/10.3892/etm.2019.8335
Pages: 1388-1392
Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Protective role of lncRNA HOXB‑AS3 in doxorubicin (DOX)‑induced cardiotoxicity and its mechanism were studied. Viability of PC and H9c2 cells treated with different doses of DOX was determined through CCK‑8 assay. Relative level of HOXB‑AS3 in DOX‑treated cardiomyocytes was detected. Regulatory effect of HOXB‑AS3 on the proliferative ability of DOX‑treated cardiomyocytes was assessed. Through dual‑luciferase reporter gene assay, the binding relationship between HOXB‑AS3 and miRNA‑875‑3p was verified. Rescue experiments were conducted to explore the role of HOXB‑AS3/miRNA‑875‑3p in influencing the proliferation of DOX‑treated cardiomyocytes. The proliferative ability of cardiomyocytes was dose‑dependently downregulated after DOX treatment. Relative level of HOXB‑AS3 was upregulated in DOX‑treated cardiomyocytes. Silence of HOXB‑AS3 in cardiomyocytes undergoing DOX treatment markedly elevated their proliferative ability. miRNA‑875‑3p was the direct target of HOXB‑AS3. Knockdown of miRNA‑875‑3p reversed the role of HOXB‑AS3 in regulating the proliferative ability of cardiomyocytes. HOXB‑AS3 protects DOX‑induced suppression in the proliferation of cardiomyocytes through targeting and downregulating miRNA‑875‑3p.

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