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Identification of differentially expressed miRNAs associated with thermal injury in epidermal stem cells based on RNA‑sequencing

  • Authors:
    • Hao‑Tian Rong
    • De‑Wu Liu

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  • Published online on: January 14, 2020     https://doi.org/10.3892/etm.2020.8448
  • Pages: 2218-2228

  • Copyright: © Rong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Current research indicates that epidermal stem cells (EpSCs) play an important role in promoting wound healing, but the mechanism of action of these cells during wound repair following thermal damage remains unclear. In the present study, the trypsin digestion method was used to isolate human EpSCs and the cells were incubated in a 51.5˚C water tank for 35 sec to construct a thermal injury model. The differentially expressed miRNAs were identified using high‑throughput sequencing technology, and bioinformatic methods were used to predict their target genes and signaling pathways that may be involved in wound repair. A total of 33 miRNAs including, hsa‑miR‑1973, hsa‑miR‑4485‑3p, hsa‑miR‑548‑5p, hsa‑miR‑212‑3p and hsa‑miR‑4461 were upregulated, whereas 21 miRNAs including, hsa‑miR‑4520‑5p, hsa‑miR‑4661‑5p, hsa‑miR‑191‑3p, hsa‑miR‑129‑5p, hsa‑miR‑147b and hsa‑miR‑6868‑3p were downregulated following thermal injury of the human EpSCs. The bioinformatic analysis indicated that the differentially expressed miRNAs are involved in biological processes such as cell proliferation and differentiation, cell growth apoptosis, cell adhesion and migration. The results showed that there is a differential expression pattern of miRNAs after thermal injury of human EpSCs and these differences are involved in the regulation of the wound healing process. These findings provide new clues for further study of the wound healing mechanism and targeted therapy.
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March-2020
Volume 19 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Rong HT and Liu DW: Identification of differentially expressed miRNAs associated with thermal injury in epidermal stem cells based on RNA‑sequencing. Exp Ther Med 19: 2218-2228, 2020.
APA
Rong, H., & Liu, D. (2020). Identification of differentially expressed miRNAs associated with thermal injury in epidermal stem cells based on RNA‑sequencing. Experimental and Therapeutic Medicine, 19, 2218-2228. https://doi.org/10.3892/etm.2020.8448
MLA
Rong, H., Liu, D."Identification of differentially expressed miRNAs associated with thermal injury in epidermal stem cells based on RNA‑sequencing". Experimental and Therapeutic Medicine 19.3 (2020): 2218-2228.
Chicago
Rong, H., Liu, D."Identification of differentially expressed miRNAs associated with thermal injury in epidermal stem cells based on RNA‑sequencing". Experimental and Therapeutic Medicine 19, no. 3 (2020): 2218-2228. https://doi.org/10.3892/etm.2020.8448