lncRNA‑ATB promotes stemness maintenance in colorectal cancer by regulating transcriptional activity of the β‑catenin pathway

Yang,Xiaojin; Tao, Hanchuan; Wang,Cheng; Chen,Weijun; Hua,Fu; Qian,Haixin

doi:10.3892/etm.2020.8558

lncRNA‑ATB promotes stemness maintenance in colorectal cancer by regulating transcriptional activity of the β‑catenin pathway

Authors:
- Xiaojin Yang
- Hanchuan Tao
- Cheng Wang
- Weijun Chen
- Fu Hua
- Haixin Qian
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Affiliations: Department of General Surgery, First Affiliated Hospital of Soochow University, Soochow, Jiangsu 215006, P.R. China, Department of Neurology, Affiliated Dongtai Hospital of Nantong University, Dongtai, Jiangsu 224200, P.R. China, Department of General Surgery, Affiliated Dongtai Hospital of Nantong University, Dongtai, Jiangsu 224200, P.R. China
Published online on: February 26, 2020 https://doi.org/10.3892/etm.2020.8558
Pages: 3097-3103
Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non‑coding RNA activated by transforming growth factor‑β (ATB) was recently reported to be involved in a wide range of physiological and pathological processes. However, the role of ATB in colorectal cancer (CRC) stemness remains unclear. In the present study, the functional role of ATB in maintaining stemness of CRC was determined using colony formation and sphere formation assays, and xenograft models. Reverse transcription‑quantitative PCR, western blotting and immunohistochemistry were performed to investigate the mechanisms underlying the effects of ATB. Knockdown of ATB impaired colony formation and sphere formation in CRC cells, accompanied by an inhibition of colon tumor growth. Further results suggested that ATB regulated the transcriptional activity of the β‑catenin pathway by inhibiting β‑catenin expression. In addition, the results confirmed the role of β‑catenin in ATB‑mediated regulation of stemness in CRC. Collectively, the results indicated that ATB is a promising therapeutic target for CRC.

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