Role of TGF‑&beta;1‑mediated epithelial‑mesenchymal transition in the pathogenesis of tympanosclerosis

Qiu,Jingjing; Wang,Yanmei; Guo,Wentao; Xu,Ling; Mou,Yakui; Cui,Limei; Han,Fengchan; Sun,Yan

doi:10.3892/etm.2020.9438

Role of TGF‑β1‑mediated epithelial‑mesenchymal transition in the pathogenesis of tympanosclerosis

Authors:
- Jingjing Qiu
- Yanmei Wang
- Wentao Guo
- Ling Xu
- Yakui Mou
- Limei Cui
- Fengchan Han
- Yan Sun
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Affiliations: Department of Otorhinolaryngology Head and Neck Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China, Department of Blood Purification, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China, Key Laboratory for Genetic Hearing Disorders in Shandong, Binzhou Medical University, Yantai, Shandong 264003, P.R. China
Published online on: November 2, 2020 https://doi.org/10.3892/etm.2020.9438
Article Number: 6
Copyright: © Qiu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to explore the role of TGF‑β1‑mediated epithelial‑mesenchymal transition (EMT) in the pathogenesis of tympanosclerosis. Sprague Dawley rats were injected with inactivated Streptococcus pneumoniae suspension to establish a rat model of tympanosclerosis. The rats were sacrificed 8 weeks after the model was established. H&E and von Kossa staining was used to observe the morphological changes of middle ear mucosa. Western blotting was used to detect the expression of TGF‑β1 and EMT‑associated proteins in the mucosa samples. Middle ear mucosal epithelial cells of rats were collected to establish a primary culture. The cultured cells were stimulated with TGF‑β1 and the expression of EMT‑associated proteins was detected by western blotting and immunofluorescence. In addition, the cells were treated with TGF‑β receptor type I/II inhibitor and the expression level of EMT‑associated proteins was detected by western blotting. Sclerotic lesions appeared on 72.4% of tympanic membranes, and marked inflammation, inflammatory cell infiltration and fibrosis were found in the middle ear mucosa of rat models of tympanosclerosis. In middle ear mucosa of rats with tympanosclerosis, the expression of mesenchymal cell markers increased and that of epithelial cell markers decreased compared with the control group. TGF‑β1 stimulated the activation of the EMT pathway in middle ear mucosal epithelial cells, resulting in an increased expression of fibronectin and N‑cadherin. In addition, a decreased expression level of EMT‑associated proteins was observed when TGF‑β1 was inhibited. In conclusion, the present study indicated that TGF‑β1‑mediated EMT may play an important role in the pathogenesis of tympanosclerosis.

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