Open Access

Identification of adhesion‑associated DNA methylation patterns in the peripheral nervous system

  • Authors:
    • Shanhuai Zuo
    • Guidong Shi
    • Jianchao Fan
    • Baoyou Fan
    • Xiaolei Zhang
    • Shen Liu
    • Yan Hao
    • Zhijian Wei
    • Xianhu Zhou
    • Shiqing Feng
  • View Affiliations

  • Published online on: November 18, 2020     https://doi.org/10.3892/etm.2020.9479
  • Article Number: 48
  • Copyright: © Zuo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Schwann cells are unique glial cells in the peripheral nervous system. These cells provide a range of cytokines and nutritional factors to maintain axons and support axonal regeneration. However, little is known concerning adhesion‑associated epigenetic changes that occur in Schwann cells after peripheral nerve injury (PNI). In the present study, adhesion‑associated DNA methylation biomarkers were assessed between normal and injury peripheral nerve. Specifically, normal Schwann cells (NSCs) and activated Schwann cells (ASCs) were obtained from adult Wistar rats. After the Schwann cells were identified, proliferation and adhesion assays were used to assess differences between NSCs and ASCs. Methylated DNA immunoprecipitation‑sequencing and bioinformatics analysis were used to identify and analyze the differentially methylated genes. Reverse transcription‑quantitative PCR was performed to assess the expression levels of adhesion‑associated genes. In the present study, the proliferation and adhesion assays demonstrated that ASCs had a more robust proliferative activity and adhesion compared with NSCs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed to identify methylation‑associated biological processes and signaling pathways. Protein‑protein interaction network analysis revealed that Fyn, Efna1, Jak2, Vav3, Flt4, Epha7, Crk, Kitlg, Ctnnb1 and Ptpn11 were potential markers for Schwann cell adhesion. The expression levels of several adhesion‑associated genes, such as vinculin, BCAR1 scaffold protein, collagen type XVIII α1 chain and integrin subunit β6, in ASCs were altered compared with those in NSCs. The current study analyzed adhesion‑associated DNA methylation patterns of Schwann cells and identified candidate genes that may potentially regulate Schwann cell adhesion in Wistar rats before and after PNI.
View Figures
View References

Related Articles

Journal Cover

January-2021
Volume 21 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zuo S, Shi G, Fan J, Fan B, Zhang X, Liu S, Hao Y, Wei Z, Zhou X, Feng S, Feng S, et al: Identification of adhesion‑associated DNA methylation patterns in the peripheral nervous system. Exp Ther Med 21: 48, 2021.
APA
Zuo, S., Shi, G., Fan, J., Fan, B., Zhang, X., Liu, S. ... Feng, S. (2021). Identification of adhesion‑associated DNA methylation patterns in the peripheral nervous system. Experimental and Therapeutic Medicine, 21, 48. https://doi.org/10.3892/etm.2020.9479
MLA
Zuo, S., Shi, G., Fan, J., Fan, B., Zhang, X., Liu, S., Hao, Y., Wei, Z., Zhou, X., Feng, S."Identification of adhesion‑associated DNA methylation patterns in the peripheral nervous system". Experimental and Therapeutic Medicine 21.1 (2021): 48.
Chicago
Zuo, S., Shi, G., Fan, J., Fan, B., Zhang, X., Liu, S., Hao, Y., Wei, Z., Zhou, X., Feng, S."Identification of adhesion‑associated DNA methylation patterns in the peripheral nervous system". Experimental and Therapeutic Medicine 21, no. 1 (2021): 48. https://doi.org/10.3892/etm.2020.9479