Role of lincRNA‑Cox2 targeting miR‑150 in regulating the viability of chondrocytes in osteoarthritis

Jiang,Meng; Xu,Kai; Ren,Huafeng; Wang,Mingmin; Hou,Ximin; Cao,Jianping

doi:10.3892/etm.2021.10232

Role of lincRNA‑Cox2 targeting miR‑150 in regulating the viability of chondrocytes in osteoarthritis

Authors:
- Meng Jiang
- Kai Xu
- Huafeng Ren
- Mingmin Wang
- Ximin Hou
- Jianping Cao
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Affiliations: Department of Orthopaedics, Qingdao No. 6 People's Hospital, Qingdao, Shandong 266033, P.R. China, Department of Functional Examination, Qingdao Haici Medical Group, Qingdao, Shandong 266033, P.R. China, Department of Anesthesiology, Qingdao No. 6 People's Hospital, Qingdao, Shandong 266033, P.R. China
Published online on: May 25, 2021 https://doi.org/10.3892/etm.2021.10232
Article Number: 800
Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Osteoarthritis (OA) is a joint disease characterised by progressive cartilage degradation and inflammation, but the detailed pathogenesis of OA remains unclear. The present study aimed to investigate the role of long intergenic non‑coding RNA (lincRNA)‑Cox2 in OA progression and the potential mechanism. An OA mouse model was used for in vivo experiments, and IL‑1β‑induced injury of mouse chondrocytes was conducted for in vitro experiments. Small interfering (si)‑Cox2 was transfected into chondrocytes to elucidate the effect of lincRNA‑Cox2 on OA. Quantitative reverse transcription PCR assays were conducted to detect the expression of lincRNA‑Cox2 and microRNA (miR)‑150. Cell proliferation and apoptosis were analysed based on an MTT assay and annexin V/propidium iodide staining, respectively. Western blotting was performed to evaluate the protein expression levels of Ki‑67, PCNA, Bax, cleaved (c)‑Caspase‑3, c‑Caspase‑9 and Wnt/β‑catenin pathway‑associated proteins in chondrocytes. High levels of lincRNA‑Cox2 were observed in cartilage tissues of the OA mouse model in vivo. In the in vitro experiments, the expression of lincRNA‑Cox2 was increased in IL‑1β‑treated chondrocytes. Knockdown of lincRNA‑Cox2 promoted the proliferation and inhibited the apoptosis of chondrocytes. Mechanistically, lincRNA‑Cox2 was found to directly target miR‑150, acting as a competing endogenous RNA, and the effect of si‑Cox2 on the proliferation and apoptosis of chondrocytes was reversed by miR‑150 inhibitors. Moreover, lincRNA‑Cox2 activated the Wnt/β‑catenin pathway to regulate chondrocyte proliferation and apoptosis. The present study demonstrated that silencing lincRNA‑Cox2 expression plays a protective role in OA by enhancing the proliferation and suppressing the apoptosis of chondrocytes, which is related to increased miR‑150 expression and activation of the Wnt/β‑catenin pathway.

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