DNCP induces the differentiation of induced pluripotent stem cells into odontoblasts by activating the Smad/p‑Smad and p38/p‑p38 signaling pathways

  • Authors:
    • Zhe Liu
    • Aiping Zhan
    • Sumeng Fan
    • Lan Liao
    • Wenwei Lian
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  • Published online on: September 24, 2021     https://doi.org/10.3892/etm.2021.10481
  • Article Number: 1361
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Abstract

In recent years, stem cells have been studied for treating tooth loss. The present study aimed to investigate the roles of dentin non‑collagen protein (DNCP)‑associated microenvironments in the differentiation of induced pluripotent stem cells (iPSCs) into dentin cells. iPSCs were cultured and identified by examining octamer‑binding transcription‑factor‑4 (Oct‑4) and sex‑determining region‑Y‑2 (Sox‑2) expression. iPSCs were differentiated by culturing DNCP‑associated microenvironments (containing specific growth factors), and they were divided into control, DNCP, DNCP+bone morphogenetic proteins (BMPs) and DNCP+Noggin (a BMP inhibitor) groups. Msh homeobox 1 (Msx‑1), dentin sialophosphoprotein (DSPP) and dentin matrix protein 1 (DMP‑1) mRNA expression was evaluated using reverse transcription‑quantitative PCR. The levels of p38, phosphorylated (p)‑p38, Smad and p‑Smad were determined by western blotting. Upon treatment with mouse embryonic fibroblasts, iPSCs‑dependent embryoid bodies (EBs) were successfully generated. iPSCs exhibited increased Oct‑4 and Sox‑2 expression. Differentiated iPSCs had higher expression levels of DSPP, DMP‑1 and Msx‑1 in the DNCP group compared with those in the control group (P<0.05). Noggin treatment significantly downregulated, while BMPs administration significantly increased the expression levels of DSPP, DMP‑1 and Msx‑1 compared with those of the DNCP group (P<0.05). The ratios of p‑p38/p38 and p‑Smad/Smad were significantly higher in the DNCP group compared with those in the control group (P<0.05). Noggin and BMPs significantly decreased ratios of p‑p38/p38, compared with those of the DNCP group (P<0.05). In conclusion, DNCP induced the differentiation of iPSCs into odontoblasts by activating the Smad/p‑Smad and p38/p‑p38 signaling pathways.
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December-2021
Volume 22 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu Z, Zhan A, Fan S, Liao L and Lian W: DNCP induces the differentiation of induced pluripotent stem cells into odontoblasts by activating the Smad/p‑Smad and p38/p‑p38 signaling pathways. Exp Ther Med 22: 1361, 2021
APA
Liu, Z., Zhan, A., Fan, S., Liao, L., & Lian, W. (2021). DNCP induces the differentiation of induced pluripotent stem cells into odontoblasts by activating the Smad/p‑Smad and p38/p‑p38 signaling pathways. Experimental and Therapeutic Medicine, 22, 1361. https://doi.org/10.3892/etm.2021.10481
MLA
Liu, Z., Zhan, A., Fan, S., Liao, L., Lian, W."DNCP induces the differentiation of induced pluripotent stem cells into odontoblasts by activating the Smad/p‑Smad and p38/p‑p38 signaling pathways". Experimental and Therapeutic Medicine 22.6 (2021): 1361.
Chicago
Liu, Z., Zhan, A., Fan, S., Liao, L., Lian, W."DNCP induces the differentiation of induced pluripotent stem cells into odontoblasts by activating the Smad/p‑Smad and p38/p‑p38 signaling pathways". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1361. https://doi.org/10.3892/etm.2021.10481