Open Access

Acidic preconditioning reduces lipopolysaccharide‑induced acute lung injury by upregulating the expression of angiotensin‑converting enzyme 2

  • Authors:
    • Guiyuan Yu
    • Yan Jiao
    • Jia-Jia Huang
    • Ming-Da Fan
    • Yu-Chen Hao
    • Ji-Zhong Han
    • Liangchao Qu
  • View Affiliations

  • Published online on: February 28, 2021     https://doi.org/10.3892/etm.2021.9879
  • Article Number: 441
  • Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Acid preconditioning (APC) through carbon dioxide inhalation can exert protective effects during acute lung injury (ALI) triggered by ischemia‑reperfusion. Angiotensin‑converting enzyme 2 (ACE2) has been identified as a receptor for severe acute respiratory syndrome coronavirus and the novel coronavirus disease‑19. Downregulation of ACE2 plays an important role in the pathogenesis of severe lung failure after viral or bacterial infections. The aim of the present study was to examine the anti‑inflammatory mechanism through which APC alleviates lipopolysaccharide (LPS)‑induced ALI in vivo and in vitro. The present results demonstrated that LPS significantly downregulated the expression of ACE2, while APC significantly reduced LPS‑induced ALI and provided beneficial effects. In addition, bioinformatics analysis indicated that microRNA (miR)‑200c‑3p directly targeted the 3'untranslated region of ACE2 and regulated the expression of ACE2 protein. LPS exposure inhibited the expression of ACE2 protein in A549 cells by upregulating the levels of miR‑200c‑3p. However, APC inhibited the upregulation of miR‑200c‑3p induced by LPS, as well as the downregulation of ACE2 protein, through the NF‑κB pathway. In conclusion, although LPS can inhibit the expression of ACE2 by upregulating the levels of miR‑200c‑3p through the NF‑κB pathway, APC inhibited this effect, thus reducing inflammation during LPS‑induced ALI.
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May-2021
Volume 21 Issue 5

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Spandidos Publications style
Yu G, Jiao Y, Huang J, Fan M, Hao Y, Han J and Qu L: Acidic preconditioning reduces lipopolysaccharide‑induced acute lung injury by upregulating the expression of angiotensin‑converting enzyme 2. Exp Ther Med 21: 441, 2021.
APA
Yu, G., Jiao, Y., Huang, J., Fan, M., Hao, Y., Han, J., & Qu, L. (2021). Acidic preconditioning reduces lipopolysaccharide‑induced acute lung injury by upregulating the expression of angiotensin‑converting enzyme 2. Experimental and Therapeutic Medicine, 21, 441. https://doi.org/10.3892/etm.2021.9879
MLA
Yu, G., Jiao, Y., Huang, J., Fan, M., Hao, Y., Han, J., Qu, L."Acidic preconditioning reduces lipopolysaccharide‑induced acute lung injury by upregulating the expression of angiotensin‑converting enzyme 2". Experimental and Therapeutic Medicine 21.5 (2021): 441.
Chicago
Yu, G., Jiao, Y., Huang, J., Fan, M., Hao, Y., Han, J., Qu, L."Acidic preconditioning reduces lipopolysaccharide‑induced acute lung injury by upregulating the expression of angiotensin‑converting enzyme 2". Experimental and Therapeutic Medicine 21, no. 5 (2021): 441. https://doi.org/10.3892/etm.2021.9879