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miR‑223 ameliorates thalamus hemorrhage‑induced central poststroke pain via targeting NLRP3 in a mouse model

  • Authors:
    • Tianfeng Huang
    • Yinggang Xiao
    • Yang Zhang
    • Cunjin Wang
    • Xiaoping Chen
    • Yong Li
    • Yali Ge
    • Ju Gao

  • View Affiliations

  • Published online on: March 28, 2022     https://doi.org/10.3892/etm.2022.11280
  • Article Number: 353

  • Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Central poststroke pain (CPSP) is a central neuropathic pain syndrome that occurs following a stroke and mainly manifests as pain and paresthesia in the body region corresponding to the brain injury area. At present, due to the lack of clinical attention given to CPSP, patients suffer from long‑term pain that seriously affects their quality of life. Current literature indicates that microRNA (miR)‑223 can impede inflammation and prevent collateral damage. The NLR family pyrin domain containing 3 (NLRP3) inflammasome induces IL‑18 and IL‑1β secretion and maturation and participates in the inflammatory response. Previous evidence has confirmed that miR‑223 can negatively regulate NLRP3 in the development of inflammatory responses. However, whether the miR‑223 targeting of NLRP3 is involved in CPSP remains unclear. In the present study, the expression of miR‑223 was detected by reverse transcription‑quantitative PCR analysis. The expression levels of NLRP3, caspase‑1, ASC, IL‑18, IL‑1β, ERK1/2, p‑ERK1/2 and GFAP were detected by western blot analysis. The results demonstrated that thalamic hemorrhagic stroke triggered by microinjection of collagenase Ⅳ (Coll IV) into the ventral posterior lateral (VPL) nucleus results in pain hypersensitivity. miR‑223 expression level were significantly reduced in the CPSP model. The expression levels of NLRP3, caspase‑1, ASC, IL‑18 and IL‑1β were significantly increased in the CPSP model. The expression level of GFAP was detected to determine astrocyte activation. The results demonstrated that astrocyte activation induced by Coll IV produced a CPSP model. The p‑ERK1/2 expression level was demonstrated to be significantly increased in the CPSP model. The introduction of an miR‑223 agomir significantly attenuated thalamic pain and significantly decreased the levels of NLRP3, caspase‑1, ASC and proinflammatory cytokines (IL‑18 and IL‑1β). Furthermore, introducing a miR‑223 antagomir into the VPL nucleus of naïve mice mimicked thalamic pain and significantly increased the levels of NLRP3, caspase‑1, ASC and proinflammatory cytokine levels (IL‑18 and IL‑1β). These results indicated that miR‑223 inhibited NLRP3 inflammasome activity (caspase‑1, NLRP3 and ASC), which ameliorated thalamus hemorrhage‑induced CPSP in mice via NLRP3 downregulation. In conclusion, these results may determine the mechanisms underlying CPSP and facilitate development of targeted therapy for CPSP.
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May-2022
Volume 23 Issue 5

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Spandidos Publications style
Huang T, Xiao Y, Zhang Y, Wang C, Chen X, Li Y, Ge Y and Gao J: miR‑223 ameliorates thalamus hemorrhage‑induced central poststroke pain via targeting NLRP3 in a mouse model. Exp Ther Med 23: 353, 2022.
APA
Huang, T., Xiao, Y., Zhang, Y., Wang, C., Chen, X., Li, Y. ... Gao, J. (2022). miR‑223 ameliorates thalamus hemorrhage‑induced central poststroke pain via targeting NLRP3 in a mouse model. Experimental and Therapeutic Medicine, 23, 353. https://doi.org/10.3892/etm.2022.11280
MLA
Huang, T., Xiao, Y., Zhang, Y., Wang, C., Chen, X., Li, Y., Ge, Y., Gao, J."miR‑223 ameliorates thalamus hemorrhage‑induced central poststroke pain via targeting NLRP3 in a mouse model". Experimental and Therapeutic Medicine 23.5 (2022): 353.
Chicago
Huang, T., Xiao, Y., Zhang, Y., Wang, C., Chen, X., Li, Y., Ge, Y., Gao, J."miR‑223 ameliorates thalamus hemorrhage‑induced central poststroke pain via targeting NLRP3 in a mouse model". Experimental and Therapeutic Medicine 23, no. 5 (2022): 353. https://doi.org/10.3892/etm.2022.11280