Open Access

FOXA1 transcription activates TFF1 to reduce 6‑OHDA‑induced dopaminergic neuron damage

  • Authors:
    • Tingting Liang
    • Ping Zhao
    • Xiao Zhang
    • Xuedan Han
    • Bo Hong
    • Lingsheng Kong
    • Huanxian Chang
    • Liyan Liu
  • View Affiliations

  • Published online on: April 5, 2022     https://doi.org/10.3892/etm.2022.11299
  • Article Number: 372
  • Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Forkhead box A1 (FOXA1) plays an important role in the central nervous system, and its loss can lead to the downregulation of tyrosine hydroxylase, which directly affects the synthesis of dopamine, thus leading to Parkinson's disease (PD). The present study aimed to explore the specific role of FOXA1 in PD. Blood samples from patients with PD were collected to determine the expression levels of FOXA1 using reverse transcription‑quantitative PCR (RT‑qPCR). In addition, mouse dopaminergic neuron MES23.5 cells were induced with 6‑hydroxydopamine (6‑OHDA) to construct an in vitro PD model in order to study the effect of FOXA1 overexpression on cell inflammation, oxidative stress and apoptosis with RT‑qPCR, assay kits and TUNEL assays, respectively. Subsequently, the expression of FOXA1 was silenced to assess the effect on the downstream mechanism. The results revealed that the expression level of FOXA1 was downregulated in patients with PD, and FOXA1 overexpression attenuated 6‑OHDA‑induced inflammation, oxidative stress and apoptosis in MES23.5 cells. Furthermore, FOXA1 could bind to the trefoil factor 1 (TFF1) promoter, and the effects of FOXA1 overexpression on cells were reversed by TFF1 silencing, indicating that TFF1 mediated the mechanism of FOXA1 overexpression in MES23.5 cells. In conclusion, following FOXA1 transcription, TFF1 expression was activated, thereby relieving 6‑OHDA‑induced cell inflammation, oxidative stress and apoptosis. The present findings suggested that FOXA1 may serve as a target for the treatment of PD.
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June-2022
Volume 23 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liang T, Zhao P, Zhang X, Han X, Hong B, Kong L, Chang H and Liu L: FOXA1 transcription activates TFF1 to reduce 6‑OHDA‑induced dopaminergic neuron damage. Exp Ther Med 23: 372, 2022.
APA
Liang, T., Zhao, P., Zhang, X., Han, X., Hong, B., Kong, L. ... Liu, L. (2022). FOXA1 transcription activates TFF1 to reduce 6‑OHDA‑induced dopaminergic neuron damage. Experimental and Therapeutic Medicine, 23, 372. https://doi.org/10.3892/etm.2022.11299
MLA
Liang, T., Zhao, P., Zhang, X., Han, X., Hong, B., Kong, L., Chang, H., Liu, L."FOXA1 transcription activates TFF1 to reduce 6‑OHDA‑induced dopaminergic neuron damage". Experimental and Therapeutic Medicine 23.6 (2022): 372.
Chicago
Liang, T., Zhao, P., Zhang, X., Han, X., Hong, B., Kong, L., Chang, H., Liu, L."FOXA1 transcription activates TFF1 to reduce 6‑OHDA‑induced dopaminergic neuron damage". Experimental and Therapeutic Medicine 23, no. 6 (2022): 372. https://doi.org/10.3892/etm.2022.11299