Open Access

Reducing polypyrimidine tract‑binding protein 1 fails to promote neuronal transdifferentiation on HT22 and mouse astrocyte cells under physiological conditions

  • Authors:
    • Qian Li
    • Wei Zhang
    • Xin-Yu Qiao
    • Chong Liu
    • Ji-Ji Dao
    • Chen-Meng Qiao
    • Chun Cui
    • Yan-Qin Shen
    • Wei-Jiang Zhao
  • View Affiliations

  • Published online on: December 19, 2023     https://doi.org/10.3892/etm.2023.12360
  • Article Number: 72
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In contrast to prior findings that have illustrated the conversion of non‑neuronal cells into functional neurons through the specific targeting of polypyrimidine tract‑binding protein 1 (PTBP1), accumulated evidence suggests the impracticality of inducing neuronal transdifferentiation through suppressing PTBP1 expression in pathological circumstances. Therefore, the present study explored the effect of knocking down PTBP1 under physiological conditions on the transdifferentiation of mouse hippocampal neuron HT22 cells and mouse astrocyte (MA) cells. A total of 20 µM negative control small interfering (si)RNA and siRNA targeting PTBP1 were transfected into HT22 and MA cells using Lipo8000™ for 3 and 5 days, respectively. The expression of early neuronal marker βIII‑Tubulin and mature neuronal markers NeuN and microtubule‑associated protein 2 (MAP2) were detected using western blotting. In addition, βIII‑tubulin, NeuN and MAP2 were labeled with immunofluorescence staining to evaluate neuronal cell differentiation in response to PTBP1 downregulation. Under physiological conditions, no significant changes in the expression of βIII‑Tubulin, NeuN and MAP2 were found after 3 and 5 days of knockdown of PTBP1 protein in both HT22 and MA cells. In addition, the immunofluorescence staining results showed no apparent transdifferentiation in maker levels and morphology. The results suggested that the knockdown of PTBP1 failed to induce neuronal differentiation under physiological conditions.
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February-2024
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Spandidos Publications style
Li Q, Zhang W, Qiao X, Liu C, Dao J, Qiao C, Cui C, Shen Y and Zhao W: Reducing polypyrimidine tract‑binding protein 1 fails to promote neuronal transdifferentiation on HT22 and mouse astrocyte cells under physiological conditions. Exp Ther Med 27: 72, 2024.
APA
Li, Q., Zhang, W., Qiao, X., Liu, C., Dao, J., Qiao, C. ... Zhao, W. (2024). Reducing polypyrimidine tract‑binding protein 1 fails to promote neuronal transdifferentiation on HT22 and mouse astrocyte cells under physiological conditions. Experimental and Therapeutic Medicine, 27, 72. https://doi.org/10.3892/etm.2023.12360
MLA
Li, Q., Zhang, W., Qiao, X., Liu, C., Dao, J., Qiao, C., Cui, C., Shen, Y., Zhao, W."Reducing polypyrimidine tract‑binding protein 1 fails to promote neuronal transdifferentiation on HT22 and mouse astrocyte cells under physiological conditions". Experimental and Therapeutic Medicine 27.2 (2024): 72.
Chicago
Li, Q., Zhang, W., Qiao, X., Liu, C., Dao, J., Qiao, C., Cui, C., Shen, Y., Zhao, W."Reducing polypyrimidine tract‑binding protein 1 fails to promote neuronal transdifferentiation on HT22 and mouse astrocyte cells under physiological conditions". Experimental and Therapeutic Medicine 27, no. 2 (2024): 72. https://doi.org/10.3892/etm.2023.12360