Identification of common genes of rhinovirus single/double‑stranded RNA‑induced asthma deterioration by bioinformatics analysis

An,Qian; Cao,Yi; Guo,Wei; Jiang,Ziyun; Luo,Hui; Liu,Hui; Zhan,Xiaodong

doi:10.3892/etm.2024.12498

Identification of common genes of rhinovirus single/double‑stranded RNA‑induced asthma deterioration by bioinformatics analysis

Authors:
- Qian An
- Yi Cao
- Wei Guo
- Ziyun Jiang
- Hui Luo
- Hui Liu
- Xiaodong Zhan
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Affiliations: Department of Respiratory and Critical Care Medicine, Wuhu Hospital of Traditional Chinese Medicine, Wuhu, Anhui 241000, P.R. China, Department of Medical Parasitology, School of Basic Medicine, Wannan Medical College, Wuhu, Anhui 241002, P.R. China, Department of Medical Parasitology, School of Basic Medicine, Wannan Medical College, Wuhu, Anhui 241002, P.R. China
Published online on: March 20, 2024 https://doi.org/10.3892/etm.2024.12498
Article Number: 210
Copyright: © An et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Rhinovirus (RV) is the most common respiratory virus affecting humans. The majority of asthma deteriorations are triggered by RV infections. However, whether the effects of RV single‑ and double‑stranded RNA on asthma deterioration have common target genes needs to be further studied. In the present study, two datasets (GSE51392 and GSE30326) were used to screen for common differentially expressed genes (cDEGs). The molecular function, signaling pathways, interaction networks, hub genes, key modules and regulatory molecules of cDEGs were systematically analyzed using online tools such as Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, STRING and NetworkAnalyst. Finally, the hub genes STAT1 and IFIH1 were verified in clinical samples using reverse transcription‑quantitative PCR (RT‑qPCR). A total of 85 cDEGs were identified. Function analysis revealed that cDEGs served an important role in the innate immune response to viruses and its regulation. Signal transducer and activator of transcription 1 (STAT1), interferon induced with helicase C domain 1 (IFIH1), interferon regulatory factor 7 (IRF7), DExD/H box helicase 58 (DDX58) and interferon‑stimulating gene 15 (ISG15) were detected to be hub genes based on the protein‑protein interactions and six topological algorithms. A key module involved in influenza A, the Toll‑like receptor signaling pathway, was identified using Cytoscape software. The hub genes were regulated by GATA‑binding factor 2 and microRNA‑146a‑5p. In addition, RT‑qPCR indicated that the expression levels of the hub genes STAT1 and IFIH1 were low during asthma deterioration compared with post‑treatment recovery samples. The present study enhanced the understanding of the mechanism of RV‑induced asthma deterioration.

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