In vitro anti-proliferative activity of alcoholic stem extract of Coscinium fenestratum in human colorectal cancer cells

Rojsanga,Piyanuch; Sukhthankar,Mugdha; Krisanapun,Chutwadee; Gritsanapan,Wandee; Buripakdi Lawson,Darunee; Baek,Seung J.

doi:10.3892/etm_00000029

In vitro anti-proliferative activity of alcoholic stem extract of Coscinium fenestratum in human colorectal cancer cells

Authors:
- Piyanuch Rojsanga
- Mugdha Sukhthankar
- Chutwadee Krisanapun
- Wandee Gritsanapan
- Darunee Buripakdi Lawson
- Seung J. Baek
View Affiliations

Affiliations: Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, Knoxville, TN, USA
Published online on: January 1, 2010 https://doi.org/10.3892/etm_00000029
Pages: 181-186

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Coscinium fenestratum (Gaertn.) Colebr. is traditionally used for the treatment of cancer, arthritis and diabetes mellitus. The purpose of this study was to determine the molecular mechanisms by which this plant shows beneficial effects. An 80% ethanolic extract of C. fenestratum (80ET) was separated by its polarity into dichloromethane (DCM) and aqueous fractions (WF), and the anti-proliferative effects of 80ET, DCM and WF were investigated. Berberine, one of the major components of C. fenestratum, was used as a control. The 80ET, DCM, WF and berberine showed anti-proliferative activity as assessed by cell growth assay. Subsequently, the pro-apoptotic proteins NAG-1 and ATF3 were increased and the cell cycle protein cyclin D1 was decreased by the extract and its fractions. Interestingly, only the DCM fraction exhibited the induction of peroxisome proliferator-activated receptor γ (PPARγ) binding activity, which represents a pro-apoptotic activity in colorectal cancer cells. The overall results of this study indicate that the extract from this plant has anti-proliferative activity through the activation of pro-apoptotic proteins and PPARγ, and may have potential as a preventive regimen in the treatment of cancer.

View Figures

View References

1.	Wattanathorn J, Uabundit N, Itarat W, Mucimapura S, Laopatarakasem P and Sripanidkulchai B: Neurotoxicity of Coscinium fenestratum stem, a medicinal plant used in traditional medicine. Food Chem Toxicol. 44:1327–1333. 2006.
2.	Punitha IS, Rajendran K and Shirwaikar A and Shirwaikar A: Alcoholic stem extract of Coscinium fenestratum regulates carbohydrate metabolism and improves antioxidant status in streptozotocin-nicotinamide induced diabetic rats. Evid Based Complement Alternat Med. 2:375–381. 2005.
3.	Baek SJ, Kim KS, Nixon JB, Wilson LC and Eling TE: Cyclooxygenase inhibitors regulate the expression of a TGF-beta superfamily member that has proapoptotic and antitumorigenic activities. Mol Pharmacol. 59:901–908. 2001.PubMed/NCBI
4.	Martinez JM, Sali T, Okazaki R, Anna C, Hollingshead M, Hose C, Monks A, Walker NJ, Baek SJ and Eling TE: Drug-induced expression of nonsteroidal anti-inflammatory drug-activated gene/macrophage inhibitory cytokine-1/prostate-derived factor, a putative tumor suppressor, inhibits tumor growth. J Pharmacol Exp Ther. 318:899–906. 2006. View Article : Google Scholar
5.	Baek SJ, Kim JS, Jackson FR, Eling TE, McEntee MF and Lee SH: Epicatechin gallate-induced expression of NAG-1 is associated with growth inhibition and apoptosis in colon cancer cells. Carcinogenesis. 25:2425–2432. 2004. View Article : Google Scholar : PubMed/NCBI
6.	Baek SJ, Wilson LC and Eling TE: Resveratrol enhances the expression of non-steroidal anti-inflammatory drug-activated gene (NAG-1) by increasing the expression of p53. Carcinogenesis. 23:425–434. 2002. View Article : Google Scholar : PubMed/NCBI
7.	Piyanuch R, Sukhthankar M, Wandee G and Baek SJ: Berberine, a natural isoquinoline alkaloid, induces NAG-1 and ATF3 expression in human colorectal cancer cells. Cancer Lett. 258:230–240. 2007. View Article : Google Scholar : PubMed/NCBI
8.	Lee SH, Cekanova M and Baek SJ: Multiple mechanisms are involved in 6-gingerol-induced cell growth arrest and apoptosis in human colorectal cancer cells. Mol Carcinog. 47:197–208. 2008. View Article : Google Scholar : PubMed/NCBI
9.	Cho KN, Sukhthankar M, Lee SH, Yoon JH and Baek SJ: Green tea catechin (-)-epicatechin gallate induces tumour suppressor protein ATF3 via eGR-1 activation. Eur J Cancer. 43:2404–2412. 2007. View Article : Google Scholar : PubMed/NCBI
10.	Lu D, Wolfgang CD and Hai T: Activating transcription factor 3, a stress-inducible gene, suppresses Ras-stimulated tumorigenesis. J Biol Chem. 281:10473–10481. 2006. View Article : Google Scholar : PubMed/NCBI
11.	Alao JP: The regulation of cyclin D1 degradation: roles in cancer development and the potential for therapeutic invention. Mol Cancer. 6:242007. View Article : Google Scholar : PubMed/NCBI
12.	Arber N, Sutter T, Miyake M, Kahn SM, Venkatraj VS, Sobrino A, Warburton D, Holt PR and Weinstein IB: Increased expression of cyclin D1 and the Rb tumor suppressor gene in c-K-ras transformed rat enterocytes. Oncogene. 12:1903–1908. 1996.PubMed/NCBI
13.	Ratschiller D, Heighway J, Gugger M, Kappeler A, Pirnia F, Schmid RA, Borner MM and Betticher DC: Cyclin D1 overexpression in bronchial epithelia of patients with lung cancer is associated with smoking and predicts survival. J Clin Oncol. 21:2085–2093. 2003. View Article : Google Scholar : PubMed/NCBI
14.	Huang JW, Shiau CW, Yang YT, Kulp SK, Chen KF, Brueggemeier RW, Shapiro CL and Chen CS: Peroxisome proliferator-activated receptor gamma-independent ablation of cyclin D1 by thiazolidinediones and their derivatives in breast cancer cells. Mol Pharmacol. 67:1342–1348. 2005. View Article : Google Scholar
15.	Deep G, Singh RP, Agarwal C, Kroll DJ and Agarwal R: Silymarin and silibinin cause G1 and G2-M cell cycle arrest via distinct circuitries in human prostate cancer PC3 cells: a comparison of flavanone silibinin with flavanolignan mixture silymarin. Oncogene. 25:1053–1069. 2006. View Article : Google Scholar : PubMed/NCBI
16.	Stepulak A, Sifringer M, Rzeski W, Endesfelder S, Gratopp A, Pohl EE, Bittigau P, Felderhoff-Mueser U, Kaindl AM, Buhrer C, Hansen HH, Stryjecka-Zimmer M, Turski L and Ikonomidou C: NMDA antagonist inhibits the extracellular signal-regulated kinase pathway and suppresses cancer growth. Proc Natl Acad Sci USA. 102:15605–15610. 2005. View Article : Google Scholar : PubMed/NCBI
17.	Barak Y, Nelson MC, Ong ES, Jones YZ, Ruiz-Lozano P, Chien KR, Koder A and Evans RM: PPAR gamma is required for placental, cardiac, and adipose tissue development. Mol Cell. 4:585–595. 1999. View Article : Google Scholar : PubMed/NCBI
18.	Bren-Mattison Y, van Putten V, Chan D, Winn R, Geraci MW and Nemenoff RA: Peroxisome proliferator-activated receptor-gamma [PPAR(gamma)] inhibits tumorigenesis by reversing the undifferentiated phenotype of metastatic non-small cell lung cancer cells (NSCLC). Oncogene. 24:1412–1422. 2005.
19.	Vignati S, Albertini V, Rinaldi A, Kwee I, Riva C, Oldrini R, Capella C, Bertoni F, Carbone GM and Catapano CV: Cellular and molecular consequences of peroxisome proliferator-activated receptor-gamma activation in ovarian cancer cells. Neoplasia. 8:851–861. 2006. View Article : Google Scholar : PubMed/NCBI
20.	Elstner E, Muller C, Koshizuka K, Williamson EA, Park D, Asou H, Shintaku P, Said JW, Heber D and Koeffler HP: Ligands for peroxisome proliferator-activated receptorgamma and retinoic acid receptor inhibit growth and induce apoptosis of human breast cancer cells in vitro and in BNX mice. Proc Natl Acad Sci USA. 95:8806–8811. 1998. View Article : Google Scholar : PubMed/NCBI
21.	Baek SJ, Wilson LC, Hsi LC and Eling TE: Troglitazone, a peroxisome proliferator-activated receptor gamma (PPAR gamma) ligand, selectively induces the early growth response-1 gene independently of PPAR gamma. A novel mechanism for its anti-tumorigenic activity. J Biol Chem. 278:5845–5853. 2003. View Article : Google Scholar
22.	Sarraf P, Mueller E, Smith WM, Wright HM, Kum JB, Aaltonen LA, De la Chapelle A, Spiegelman BM and Eng C: Loss-of-function mutations in PPAR gamma associated with human colon cancer. Mol Cell. 3:799–804. 1999. View Article : Google Scholar : PubMed/NCBI
23.	Cekanova M, Yuan JS, Li X, Kim K and Baek SJ: Gene alterations by peroxisome proliferator-activated receptor gamma agonists in human colorectal cancer cells. Int J Oncol. 32:809–819. 2008.PubMed/NCBI
24.	Yamaguchi K, Lee SH, Eling TE and Baek SJ: A novel peroxisome proliferator-activated receptor gamma ligand, MCC-555, induces apoptosis via posttranscriptional regulation of NAG-1 in colorectal cancer cells. Mol Cancer Ther. 5:1352–1361. 2006. View Article : Google Scholar
25.	Rungsimakan S: Pharmacognostic Properties of Khamin Khruea. Department of Pharmacognosy, Chulalongkorn University; Bangkok: pp. 1882001
26.	Rojsanga P, Gritsanapan W and Suntornsuk L: Determination of berberine content in the stem extracts of Coscinium fenestratum by TLC densitometry. Med Princ Pract. 15:373–378. 2006. View Article : Google Scholar : PubMed/NCBI
27.	Wongcome T, Panthong A, Jesadanont S, Kanjanapothi D, Taesotikul T and Lertprasertsuke N: Hypotensive effect and toxicology of the extract from Coscinium fenestratum (Gaertn.) Colebr. J Ethnopharmacol. 111:468–475. 2007. View Article : Google Scholar : PubMed/NCBI
28.	Malhotra S, Taneja SC and Dhar KL: Minor alkaloids from Coscinium fenestatum. Phytochemistry. 28:1998–1999. 1989. View Article : Google Scholar
29.	Pinho PMM, Pinto MMM, Kijjoa A, Pharadai K, Diaz JG and HerZ W: Protoberberine alkaloids from Coscinium fenestratum. Phytochemistry. 31:1403–1407. 1992. View Article : Google Scholar
30.	Siwon J, Verpoorte R, van Essen GFA and Baerheim Svendsen A: Studies on Indonesian medicinal plants III. The alkaloids of Coscinium fenestratum. Planta Med. 38:24–32. 1980. View Article : Google Scholar
31.	Ueda JY, Tezuka Y, Banskota AH, Le Tran Q, Tran QK, Harimaya Y, Saiki I and Kadota S: Antiproliferative activity of Vietnamese medicinal plants. Biol Pharm Bull. 25:753–760. 2002. View Article : Google Scholar : PubMed/NCBI
32.	Baek SJ, Okazaki R, Lee SH, Martinez J, Kim JS, Yamaguchi K, Mishina Y, Martin DW, Shoieb A, McEntee MF and Eling TE: Nonsteroidal anti-inflammatory drug-activated gene-1 over expression in transgenic mice suppresses intestinal neoplasia. Gastroenterology. 131:1553–1560. 2006. View Article : Google Scholar : PubMed/NCBI
33.	Morgan DO: Principles of CDK regulation. Nature. 374:131–134. 1995. View Article : Google Scholar : PubMed/NCBI
34.	Shukla S and Gupta S: Apigenin-induced cell cycle arrest is mediated by modulation of MAPK, PI3K-Akt and loss of cyclin D1 associated retinoblastoma dephosphorylation in human prostate cancer cells. Cell Cycle. 6:1102–1114. 2007. View Article : Google Scholar
35.	Lim YC, Lee SH, Song MH, Yamaguchi K, Yoon JH, Choi EC and Baek SJ: Growth inhibition and apoptosis by (-)-epicatechin gallate are mediated by cyclin D1 suppression in head and neck squamous carcinoma cells. Eur J Cancer. 42:3260–3266. 2006. View Article : Google Scholar : PubMed/NCBI
36.	Baek SJ, Wilson LC and Eling TE: Resveratrol enhances the expression of non-steroidal anti-inflammatory drug-activated gene (NAG-1) by increasing the expression of p53. Carcinogenesis. 23:425–432. 2002. View Article : Google Scholar : PubMed/NCBI
37.	Yan C, Jamaluddin MS, Aggarwal B, Myers J and Boyd DD: Gene expression profiling identifies activating transcription factor 3 as a novel contributor to the proapoptotic effect of curcumin. Mol Cancer Ther. 4:233–241. 2005.PubMed/NCBI
38.	Yin J, Hu R, Chen M, Tang J, Li F, Yang Y and Chen J: Effects of berberine on glucose metabolism in vitro. Metabolism. 51:1439–1443. 2002. View Article : Google Scholar : PubMed/NCBI