Regucalcin increases superoxide dismutase activity in the heart cytosol of normal and regucalcin transgenic rats.

  • Authors:
    • Emiko Ichikawa
    • Masayoshi Yamaguchi
  • View Affiliations

  • Published online on: October 1, 2004     https://doi.org/10.3892/ijmm.14.4.691
  • Pages: 691-696
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Abstract

The effect of regucalcin, a regulatory protein in the intracellular signaling system, on superoxide dismutase (SOD) activity in the heart cytosol of normal rats and regucalcin transgenic (TG) rats was investigated. The addition of regucalcin (10(-10) to 10(-8) M) with a physiologic concentration in the enzyme reaction mixture containing the heart cytosol obtained from normal rats caused a significant increase in SOD activity, indicating that regucalcin directly activates the enzyme. The effect of regucalcin (10(-8) M) in increasing SOD activity was not seen in the presence of dithiothreitol (DTT; 0.1 or 1.0 mM), a protecting reagent for sulfhydryl group, or N-ethylmaleimide (NEM; 0.1 or 1.0 mM), a modifying reagent for sulfhydryl group, in the reaction mixture, indicating that regucalcin does not affect the sulfhydryl group. The addition of zinc sulfate (10(-6) to 10(-4) M) in the reaction mixture did not cause a significant change in SOD activity, while the enzyme activity was markedly decreased in the presence of cupric sulfate (10(-6) to 10(-4) M). The activatory effect of regucalcin (10(-8) M) on SOD was seen in the presence of zinc (10(-4) M), while not observed in the presence of copper (10(-4) M). Moreover, SOD activity was significantly enhanced in the heart cytosol of regucalcin TG rats as compared with that of normal rats. This study demonstrates that regucalcin increases SOD activity in the heart cytosol of rats, and that its effect is not related to the sulfhydryl group of enzymes.

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October 2004
Volume 14 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ichikawa E and Yamaguchi M: Regucalcin increases superoxide dismutase activity in the heart cytosol of normal and regucalcin transgenic rats.. Int J Mol Med 14: 691-696, 2004.
APA
Ichikawa, E., & Yamaguchi, M. (2004). Regucalcin increases superoxide dismutase activity in the heart cytosol of normal and regucalcin transgenic rats.. International Journal of Molecular Medicine, 14, 691-696. https://doi.org/10.3892/ijmm.14.4.691
MLA
Ichikawa, E., Yamaguchi, M."Regucalcin increases superoxide dismutase activity in the heart cytosol of normal and regucalcin transgenic rats.". International Journal of Molecular Medicine 14.4 (2004): 691-696.
Chicago
Ichikawa, E., Yamaguchi, M."Regucalcin increases superoxide dismutase activity in the heart cytosol of normal and regucalcin transgenic rats.". International Journal of Molecular Medicine 14, no. 4 (2004): 691-696. https://doi.org/10.3892/ijmm.14.4.691