Quantitative detection of telomerase activity by real-time TRAP assay in the body fluids of cancer patients

  • Authors:
    • Woo Young Shim
    • Kyu Hyun Park
    • Hei-Chul Jeung
    • Yong Tae Kim
    • Tae Soo Kim
    • Woo Jin Hyung
    • Sung Hwan An
    • Sang Hwa Yang
    • Sung Hoon Noh
    • Hyun Cheol Chung
    • Sun Young Rha
  • View Affiliations

  • Published online on: November 1, 2005     https://doi.org/10.3892/ijmm.16.5.857
  • Pages: 857-863
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Abstract

Real-time TRAP assay was developed to improve the sensitivity and quantitative detection of telomerase activity in the body fluids of cancer patients. The sensitivity and clinical significance of the real-time TRAP assay was investigated. Real-time PCR protocol was modified by using ACX primer and SYBR green mixture from the process of TS primer extension. Real-time TRAP showed high correlation (r2=0.843, p=0.001) and sensitivity (25 times higher) compared to conventional TRAP. Of 164 samples, there were 8 positives in cytology (4.9%), 7 (4.3%) using the conventional TRAP, and 41 (25%) using real-time TRAP. In cytology positive samples, real-time TRAP showed a higher positivity than conventional TRAP (75% vs 63%) suggesting a higher sensitivity in the body fluids. There was a tendency towards a longer progression-free duration in telomerase negative patients than in positive patients, as determined by conventional and real-time TRAP. The diagnostic interval between the first positivity documentation and clinical progression was short in the order of real-time TRAP, conventional TRAP and cytology. In conclusion, real-time TRAP assay can detect telomerase activity at an earlier phase of cancer progression and can replace conventional TRAP assay for detecting the telomerase activity in body fluids.

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November 2005
Volume 16 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Shim WY, Park KH, Jeung H, Kim YT, Kim TS, Hyung WJ, An SH, Yang SH, Noh SH, Chung HC, Chung HC, et al: Quantitative detection of telomerase activity by real-time TRAP assay in the body fluids of cancer patients. Int J Mol Med 16: 857-863, 2005.
APA
Shim, W.Y., Park, K.H., Jeung, H., Kim, Y.T., Kim, T.S., Hyung, W.J. ... Rha, S.Y. (2005). Quantitative detection of telomerase activity by real-time TRAP assay in the body fluids of cancer patients. International Journal of Molecular Medicine, 16, 857-863. https://doi.org/10.3892/ijmm.16.5.857
MLA
Shim, W. Y., Park, K. H., Jeung, H., Kim, Y. T., Kim, T. S., Hyung, W. J., An, S. H., Yang, S. H., Noh, S. H., Chung, H. C., Rha, S. Y."Quantitative detection of telomerase activity by real-time TRAP assay in the body fluids of cancer patients". International Journal of Molecular Medicine 16.5 (2005): 857-863.
Chicago
Shim, W. Y., Park, K. H., Jeung, H., Kim, Y. T., Kim, T. S., Hyung, W. J., An, S. H., Yang, S. H., Noh, S. H., Chung, H. C., Rha, S. Y."Quantitative detection of telomerase activity by real-time TRAP assay in the body fluids of cancer patients". International Journal of Molecular Medicine 16, no. 5 (2005): 857-863. https://doi.org/10.3892/ijmm.16.5.857