Comano's (Trentino) thermal water interferes with the expression and secretion of vascular endothelial growth factor-A protein isoforms by cultured human psoriatic keratinocytes: A potential mechanism of its anti-psoriatic action

  • Authors:
    • Anna Chiarini
    • Ilaria Dal Pra
    • Raffaella Pacchiana
    • Lia Menapace
    • Giuseppe Zumiani
    • Mauro Zanoni
    • Ubaldo Armato
  • View Affiliations

  • Published online on: July 1, 2006     https://doi.org/10.3892/ijmm.18.1.17
  • Pages: 17-25
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Abstract

Thermal balneotherapy with Comano spa's water (CW; Trentino, Italy) is used for psoriasis and other skin disorders but the mechanism(s) of action of this hypotonic water are unknown. Since skin psoriatic manifestations are thought to be angiogenesis-dependent, we assessed CW's effects on the expression and release of VEGF-A protein isoforms by cultured human lesional keratinocytes isolated from skin biopsies performed in 9 patients. Confluent, psoriatic keratino-cytes were exposed for 11 days to DMEM, whose chemicals had been dissolved in either deionised water (DW-DMEM, controls) or CW (CW-DMEM, treated cells). As detected by Western immunoblotting (WB), incubation in CW-DMEM elicited, with respect to DW-DMEM, an increase in intra-cellular and/or cell-bound L-VEGF-A189 and L-VEGF-A165 48 kDa protein isoforms with no concurrent change in L-VEGF-A121 and L-VEGF-A165 45 kDa proteins. Moreover, WB analysis of the secreted VEGF-A (sVEGF-A) proteins showed that the 20 and 15 kDa bands corresponding to different VEGF-A isoforms were directly and remarkably reduced in keratinocyte-conditioned CW-DMEM vs. DW-DMEM. Thus, CW interferes with VEGF-A isoform expression and secretion by the psoriatic keratinocytes. These effects would reduce all VEGF-A-mediated angiogenic, vessel permeabilising, and chemotactic effects, thereby at least in part explaining the beneficial actions of CW balneotherapy on the clinical manifestations of psoriasis.

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July 2006
Volume 18 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Chiarini A, Dal Pra I, Pacchiana R, Menapace L, Zumiani G, Zanoni M and Armato U: Comano's (Trentino) thermal water interferes with the expression and secretion of vascular endothelial growth factor-A protein isoforms by cultured human psoriatic keratinocytes: A potential mechanism of its anti-psoriatic action. Int J Mol Med 18: 17-25, 2006.
APA
Chiarini, A., Dal Pra, I., Pacchiana, R., Menapace, L., Zumiani, G., Zanoni, M., & Armato, U. (2006). Comano's (Trentino) thermal water interferes with the expression and secretion of vascular endothelial growth factor-A protein isoforms by cultured human psoriatic keratinocytes: A potential mechanism of its anti-psoriatic action. International Journal of Molecular Medicine, 18, 17-25. https://doi.org/10.3892/ijmm.18.1.17
MLA
Chiarini, A., Dal Pra, I., Pacchiana, R., Menapace, L., Zumiani, G., Zanoni, M., Armato, U."Comano's (Trentino) thermal water interferes with the expression and secretion of vascular endothelial growth factor-A protein isoforms by cultured human psoriatic keratinocytes: A potential mechanism of its anti-psoriatic action". International Journal of Molecular Medicine 18.1 (2006): 17-25.
Chicago
Chiarini, A., Dal Pra, I., Pacchiana, R., Menapace, L., Zumiani, G., Zanoni, M., Armato, U."Comano's (Trentino) thermal water interferes with the expression and secretion of vascular endothelial growth factor-A protein isoforms by cultured human psoriatic keratinocytes: A potential mechanism of its anti-psoriatic action". International Journal of Molecular Medicine 18, no. 1 (2006): 17-25. https://doi.org/10.3892/ijmm.18.1.17