The gene expression and activity of calpains and the muscle wasting-associated ubiquitin ligases, atrogin-1 and MuRF1, are not altered in patients with primary hyperparathyroidism

  • Authors:
    • Amy Evenson
    • Jamie Mitchell
    • Wei Wei
    • Vitaliy Poylin
    • Sareh Parangi
    • Per-Olof Hasselgren
  • View Affiliations

  • Published online on: September 1, 2006     https://doi.org/10.3892/ijmm.18.3.471
  • Pages: 471-475
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Abstract

Hyperparathyroidism (HPT) can be associated with muscle atrophy and weakness. Muscle atrophy is typically caused by increased muscle protein breakdown. The influence of HPT on calpains and the ubiquitin-proteasome pathway, which are important regulators of muscle proteolysis, is not yet known. We examined the expression in skeletal muscle of μ- and m-calpain and the ubiquitin ligases, atrogin-1 and MuRF1, in patients with primary HPT. A biopsy was obtained from the sternohyoid muscle in patients undergoing surgery for primary HPT (n=8) and in normocalcemic control patients undergoing thyroid surgery (n=11). mRNA levels for atrogin-1, MuRF1 and the calcium-regulated proteases, μ- and m-calpain, were determined by real-time PCR. Calpain activity was measured using the calpain-specific substrate, BODIPY-FL-casein, and by zymography. Serum calcium was 11.4±0.46 and 9.5±0.10 mg/dl in HPT and control patients, respectively (p<0.01). The corresponding phosphate levels were 2.7±0.2 and 3.6±0.1 mg/dl (p<0.05). Parathyroid hormone serum concentration was 286±103 pg/ml (range, 77-946 pg/ml) in patients with HPT and was not measured in control patients. There were no significant differences in mRNA levels for atrogin-1, MuRF1, μ- or m-calpain and in calpain activity between HPT and control patients. The results suggest that the ubiquitin-proteasome and calpain systems are not activated in skeletal muscle in patients with primary HPT, at least not in patients with moderate hypercalcemia.

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September 2006
Volume 18 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Evenson A, Mitchell J, Wei W, Poylin V, Parangi S and Hasselgren P: The gene expression and activity of calpains and the muscle wasting-associated ubiquitin ligases, atrogin-1 and MuRF1, are not altered in patients with primary hyperparathyroidism. Int J Mol Med 18: 471-475, 2006.
APA
Evenson, A., Mitchell, J., Wei, W., Poylin, V., Parangi, S., & Hasselgren, P. (2006). The gene expression and activity of calpains and the muscle wasting-associated ubiquitin ligases, atrogin-1 and MuRF1, are not altered in patients with primary hyperparathyroidism. International Journal of Molecular Medicine, 18, 471-475. https://doi.org/10.3892/ijmm.18.3.471
MLA
Evenson, A., Mitchell, J., Wei, W., Poylin, V., Parangi, S., Hasselgren, P."The gene expression and activity of calpains and the muscle wasting-associated ubiquitin ligases, atrogin-1 and MuRF1, are not altered in patients with primary hyperparathyroidism". International Journal of Molecular Medicine 18.3 (2006): 471-475.
Chicago
Evenson, A., Mitchell, J., Wei, W., Poylin, V., Parangi, S., Hasselgren, P."The gene expression and activity of calpains and the muscle wasting-associated ubiquitin ligases, atrogin-1 and MuRF1, are not altered in patients with primary hyperparathyroidism". International Journal of Molecular Medicine 18, no. 3 (2006): 471-475. https://doi.org/10.3892/ijmm.18.3.471