GATA binding protein 3 overexpression and suppression significantly contribute to the regulation of allergic skin inflammation

  • Authors:
    • Chang Jun Bae
    • Jae Won Lee
    • Sun Bo Shim
    • Seung Wan Jee
    • Su Hae Lee
    • Jong Min Woo
    • Chang Kyu Lee
    • Dae Youn Hwang
  • View Affiliations

  • Published online on: April 28, 2011     https://doi.org/10.3892/ijmm.2011.685
  • Pages: 171-179
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Abstract

GATA binding protein 3 (GATA3) is a key molecule regulating the balance in the ratio of type 1 helper T (Th1) cells to type 2 helper T (Th2) cells, which is thought to be indicative of the pathogenesis of allergic diseases such as asthma and atopic dermatitis. The aim of this study was to investigate the role of GATA3 in allergic skin inflammation. Transgenic (Tg) mice overexpressing human GATA3 (hGATA3) were produced by the microinjection of pCMV/hGATA3 constructs into fertilized mouse eggs. The hGATA3 gene was successfully expressed at the protein level in the lymph node and thymus of CMV/hGATA3 transfected cells and Tg mice. CMV/hGATA3 Tg mice showed a significant increase in the allergic skin inflammation response such as ear thickness, draining auricular lymph node (aLN) weight, epidermal thickness, inflammatory cell number and Th2 immunoglobulin (Ig) concentration compared to wild-type (WT) mice after phthalic anhydride (PA) treatment. Furthermore, the secretion of Th2 type cytokines was increased by PA treatment in CMV/hGATA3 Tg mice, while the secretion of Th1 type cytokine was suppressed under the same conditions. However, the increased levels of Th2 type cytokines in CMV/hGATA3 Tg mice were almost recovered by the down-regulation of GATA3 expression with D-pinitol treatment. Therefore, these findings suggest that GATA3 could be considered as a potential target regulating the mechanism responsible for the differences in allergic skin inflammation.

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August 2011
Volume 28 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Bae CJ, Lee JW, Shim SB, Jee SW, Lee SH, Woo JM, Lee CK and Hwang DY: GATA binding protein 3 overexpression and suppression significantly contribute to the regulation of allergic skin inflammation. Int J Mol Med 28: 171-179, 2011.
APA
Bae, C.J., Lee, J.W., Shim, S.B., Jee, S.W., Lee, S.H., Woo, J.M. ... Hwang, D.Y. (2011). GATA binding protein 3 overexpression and suppression significantly contribute to the regulation of allergic skin inflammation. International Journal of Molecular Medicine, 28, 171-179. https://doi.org/10.3892/ijmm.2011.685
MLA
Bae, C. J., Lee, J. W., Shim, S. B., Jee, S. W., Lee, S. H., Woo, J. M., Lee, C. K., Hwang, D. Y."GATA binding protein 3 overexpression and suppression significantly contribute to the regulation of allergic skin inflammation". International Journal of Molecular Medicine 28.2 (2011): 171-179.
Chicago
Bae, C. J., Lee, J. W., Shim, S. B., Jee, S. W., Lee, S. H., Woo, J. M., Lee, C. K., Hwang, D. Y."GATA binding protein 3 overexpression and suppression significantly contribute to the regulation of allergic skin inflammation". International Journal of Molecular Medicine 28, no. 2 (2011): 171-179. https://doi.org/10.3892/ijmm.2011.685