Impact of single-dose application of TGF-β, copper peptide, stanozolol and ascorbic acid in hydrogel on midline laparatomy wound healing in a diabetic mouse model

Konerding,Moritz  A.; Ziebart,Thomas; Wolloscheck,Tanja; Wellmann,Axel; Ackermann,Maximilian

doi:10.3892/ijmm.2012.1005

Impact of single-dose application of TGF-β, copper peptide, stanozolol and ascorbic acid in hydrogel on midline laparatomy wound healing in a diabetic mouse model

Authors:
- Moritz A. Konerding
- Thomas Ziebart
- Tanja Wolloscheck
- Axel Wellmann
- Maximilian Ackermann
View Affiliations

Affiliations: Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg University Mainz, D-55126 Mainz, Germany, Institute of Pathology Celle, D-29222 Celle, Germany
Published online on: May 18, 2012 https://doi.org/10.3892/ijmm.2012.1005
Pages: 271-276

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Despite numerous advances and improvements in surgical techniques the incidence of incisional hernias after laparotomy remains high. The aim of this study was to investigate possible effects of single application of ascorbic acid, stanozolol, a synthetic anabolic steroid, copper peptide and transforming growth factor-β (TGF-β) on laparotomy wound healing in an incisional wound model in diabetic mice. After diabetes induction with streptozotozin in Balb-c mice, midline laparatomies were carried out. Closure of the linea alba was followed by single-dose application of the agents dissolved in a hydrogel before skin closure. The functional outcome was assessed in terms of maximum tensile strength. In addition, vessel densities, collagen contents and proliferation, were measured. The breaking strength of the skin 14 days after surgery was significantly higher in ascorbic acid (ΑΑ)-treated incisional wounds, whereas the other agents did not show a significantly better functional outcome. No significant differences were seen in vessel densities. Collagen type III contents was higher in the ΑΑ-treated animals, whereas the percentage of Ki67-positive nuclei was lower compared to the other groups. These data underline the positive effect of topically applied ascorbic acid in wound healing.

View Figures

View References

1.	J HöerM AnurovS TitkovaU KlingeC TönsA OttingerV SchumpelikInfluence of suture material and suture technique on collagen fibril diameters in midline laparotomiesEur Surg Res32359367200011182620
2.	TA SantoraJJ RoslynIncisional herniaSurg Clin North Am735575701993
3.	EJ GravesBS GiullumDetailed diagnoses and procedures, National hospital discharge survey, 1995Vital Health Stat13114619979429338
4.	D LangemoJ AndersonD HansonS HunterP ThompsonME PosthauerNutritional considerations in wound careAdv Skin Wound Care19297303200610.1097/00129334-200607000-0000716885642
5.	RE GiuntaT HolzbachC TaskovPS HolmMA KonerdingD SchamsE BiemerB GänsbacherAdVEGF165 gene transfer increases survival in overdimensioned skin flapsJ Gene Med7297306200510.1002/jgm.67515515117
6.	M ValluruCA StatonMW ReedNJ BrownTransforming growth factor beta and endoglin signaling orchestrate wound healingFront Physiol28999201110.3389/fphys.2011.0008922164144
7.	G BorkowJ GabbayR DardikAI EidelmanY LavieY GrunfeldS IkherM HuszarRC ZatcoffM MarikovskyMolecular mechanisms of enhanced wound healing by copper oxide-impregnated dressingsWound Repair Regen18266275201010.1111/j.1524-475X.2010.00573.x20409151
8.	M SumitraP ManikandanVS GayathriP MahendranL SugunaEmblica officinalis exerts wound healing action through upregulation of collagen and extracellular signal-regulated kinases (ERK1/2)Wound Repair Regen1799107200910.1111/j.1524-475X.2008.00446.x19152656
9.	A RümelinT HumbertO LuhkerA DrescherU FauthMetabolic clearance of the antioxidant ascorbic acid in surgical patientsJ Surg Res1294651200516085104
10.	GC JagetiaGK RajanikantKV Mallikarjun RaoAscorbic acid increases healing of excision wounds of mice whole body exposed to different doses of gamma-radiationBurns33484494200710.1016/j.burns.2006.08.02517223272
11.	T WolloscheckA GaumannA TerzicA HeintzT JungingerMA KonerdingInguinal hernia: measurement of the biomechanics of the lower abdominal wall and the inguinal canalHernia8233241200410.1007/s10029-004-0224-715098100
12.	LA IsraelssonT JonssonSuture length to wound length ratio and healing of midline laparotomy incisionsBr J Surg8012841286199310.1002/bjs.18008010208242299
13.	JJ HoerK JungeA SchachtruppU KlingeV SchumpelickInfluence of laparotomy closure technique on collagen synthesis in the incisional regionHernia69398200210.1007/s10029-002-0070-412209295
14.	J WissingTJ van VroonhovenME SchattenkerkHF VeenRJ PonsenJ JeekelFascia closure after midline laparotomy: results of a randomized trialBr J Surg74738741198710.1002/bjs.18007408313307992
15.	C Gutiérrez de la PenaC Medina AchiricaE Dominguez-AdameJ Medina DiézPrimary closure of laparotomies with high risk of incisional hernia using prosthetic material: analysis of usefulnessHernia7134136200312687426
16.	TS de Vries ReilinghD van GeldereB LangenhorstD de JongGJ van der WiltH van GoorRP BleichrodtRepair of large midline incisional hernias with polypropylene mesh: comparison of three operative techniquesHernia85699200414586775
17.	DA DubayX WangMA KuhnMC RobsonMG FranzThe prevention of incisional hernia formation using a delayed-release polymer of basic fibroblast growth factorAnn Surg240179186200410.1097/01.sla.0000131576.12153.ab15213634
18.	JL Wilkinson-BerkaEL FletcherAngiotensin and bradykinin: targets for the treatment of vascular and neuroglial pathology in diabetic retinopathyCurr Pharm Des1033133330200410.2174/138161204338317915544518
19.	V FalangaWound healing and its impairment in the diabetic footLancet36617361743200510.1016/S0140-6736(05)67700-816291068
20.	D AronsonEJ RayfieldHow hyperglycemia promotes atherosclerosis: Molecular mechanismsCardiovasc Diabetol1110200210.1186/1475-2840-1-112119059
21.	M AckermannT WolloscheckA WellmannVW LiWW LiMA KonerdingPriming with a combination of proangiogenic growth factors improves wound healing in normoglycemic miceInt J Mol Med27647653201121373751
22.	M AckermannT WolloscheckA WellmannVW LiWW LiMA KonerdingPriming with a combination of proangiogenic growth factors enhances wound healing in streptozotocin-induced diabetes in miceEur Surg Res478189201110.1159/00032814321720165
23.	JK WrightAJ SmithTE CawstonBL HazlemanThe effect of the anabolic steroid, stanozolol, on the production of procollagenase by human synovial and skin fibroblasts in vitroAgents Actions28279282198910.1007/BF019674152556901
24.	AJ EllisTE CawstonEJ MackieThe differential effects of stanozolol on human skin and synovial fibroblasts in vitro: DNA synthesis and receptor bindingAgents Actions413743199410.1007/BF019863918079819
25.	V FalangaAS GreenbergL ZhouSM OchoaAB RobertsA FalabellaY YamaguchiStimulation of collagen synthesis by the anabolic steroid stanozololJ Invest Dermatol11111931197199810.1046/j.1523-1747.1998.00431.x9856839
26.	B KaplanB GonulS DincerFN Dincer KayaA BabulRelationships between tensile strength, ascorbic acid, hydroxyproline, and zinc levels of rabbit full-thickness incision wound healingSurg Today2004747751200415338346
27.	KJ DesnevesBE TodorovicA CassarTC CroweTreatment with supplementary arginine, ascorbic acid and zinc in patients with pressure ulcers: a randomised controlled trialClin Nutr24979987200510.1016/j.clnu.2005.06.01116297506
28.	XJ WangG HanP OwensY SiddiquiAG LiRole of TGF beta-mediated inflammation in cutaneous wound healingJ Investig Dermatol Symp Proc11112117200610.1038/sj.jidsymp.565000417069018
29.	U StumpfM MichaelisD KlassertJ CinatlJ AltrichterJ WindolfJ HergenrötherM ScholzSelection of proangiogenic ascorbate derivatives and their exploitation in a novel drug-releasing system for wound healingWound Repair Regen19597607201110.1111/j.1524-475X.2011.00718.x22092798