microRNA expression profiling of the developing mouse heart

Cao,Li; Kong,Li-Ping; Yu,Zhang-Bin; Han,Shu-Ping; Bai,Yun-Fei; Zhu,Jingai; Hu,Xiaoshan; Zhu,Chun; Zhu,Shasha; Guo,Xi-Rong

doi:10.3892/ijmm.2012.1092

microRNA expression profiling of the developing mouse heart

Authors:
- Li Cao
- Li-Ping Kong
- Zhang-Bin Yu
- Shu-Ping Han
- Yun-Fei Bai
- Jingai Zhu
- Xiaoshan Hu
- Chun Zhu
- Shasha Zhu
- Xi-Rong Guo
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Affiliations: State Key Laboratory of Reproductive Medicine, Department of Ultrasound, Nanjing Maternal and Child Health Hospital, Nanjing Medical University, Nanjing 210029, P.R. China, State Key Laboratory of Reproductive Medicine, Department of Pediatrics, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, P.R. China, State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P.R. China
Published online on: August 9, 2012 https://doi.org/10.3892/ijmm.2012.1092
Pages: 1095-1104

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Abstract

microRNAs (miRNAs) play an important role in regulating normal organ physiology and development. Many miRNAs show spatially and temporally restricted expression patterns during embryogenesis and organogenesis. This study aimed to characterize the miRNA profile of the fetal mouse heart at 4 key time-points [embryonic day (E)12.5, E14.5, E16.5 and E18.5] in its development, by performing a sequencing by oligonucleotide ligation and detection (SOLiD) miRNA screen. The 4 time-points were designated as groups M1 (E18.5), M2 (E16.5), M3 (E14.5) and M4 (E12.5). miRNAs found to have consistent fold-changes of >2.0) between the 4 time-points were selected for further analysis. Ten miRNAs (mmu-miR-23b, mmu-miR-24, mmu-miR-23a, mmu-miR-375, mmu-miR-29a, mmu-miR-93, mmu-miR-21, mmu-miR-25, mmu-let-7b and mmu-miR-27b) that were the most highly expressed in the 4 groups, including the percentage >1% of total read counts, were identified. No miRNA was consistently downregulated or upregulated. There were 16 differentially expressed miRNAs between the later development group (M1+M2) and the early development group (M3+M4), which were validated by quantitative real-time PCR. Several members of the let-7 miRNA cluster (mmu-let-7a/7d/7e/7f) were upregulated in the later development group compared with the early development group. A network analysis of the predicted targets of mmu-let-7a/7d/7e/7f identified 5 target genes (FOXP1, TBX5, HAND1, AKT2 and PPARGC1A), known to be involved in cardiac development. Therefore, this study identified several miRNAs that are abundantly expressed in the developing heart, several of which are differentially expressed in the 4 time-points studied. Findings of this analysis may thus clarify the mechanisms of normal heart development and provide a physiological basis for future studies on congenital heart disease.

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