Cis-guggulsterone inhibits the IKK/NF-κB pathway, whereas trans-guggulsterone inhibits MAPK/AP-1 in MCF‑7 breast cancer cells: Guggulsterone regulates MMP‑9 expression in an isomer-specific manner

  • Authors:
    • Eun-Mi Noh
    • Eun Yong Chung
    • Hyun Jo Youn
    • Sung Hoo Jung
    • Hyun Hur
    • Young-Rae Lee
    • Jong-Suk Kim
  • View Affiliations

  • Published online on: December 14, 2012     https://doi.org/10.3892/ijmm.2012.1214
  • Pages: 393-399
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Νuclear factor-κB (NF-κB) and activator protein-1 (AP-1) are major transcription factors that have been associated with breast cancer metastasis by inducing matrix metalloproteinase-9 (MMP-9) expression. In this study, we investigated the inhibitory effects of guggulsterone isomers (cis or trans) on 12-O-tetradecanoylpho-bol-13-acetate (TPA)-induced MMP-9 expression. Cis-guggulsterone inhibited TPA-induced MMP expression by blocking IκB kinase (IKK)/NF-κB signaling, whereas trans-guggulsterone blocked mitogen-activated protein kinase (MAPK)/AP-1 signaling. Cis-guggulsterone was more potent than trans-guggulsterone in the inhibition of TPA-induced MMP-9 expression and invasion of MCF-7 cells. Furthermore, we found that the combination of these isomers exerted an additive effect on the inhibition of MCF-7 cell invasion. These results suggest that guggulsterone isomers downregulate MMP-9 expression and tumor cell invasion through the isomer-specific suppression of IKK/NF-κB and MAPK/AP-1 activation. In addition, the suppression of MMP-9 expression correlated well with the inhibition of cell invasion.
View Figures
View References

Related Articles

Journal Cover

February 2013
Volume 31 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Noh E, Chung EY, Youn HJ, Jung SH, Hur H, Lee Y and Kim J: Cis-guggulsterone inhibits the IKK/NF-κB pathway, whereas trans-guggulsterone inhibits MAPK/AP-1 in MCF‑7 breast cancer cells: Guggulsterone regulates MMP‑9 expression in an isomer-specific manner. Int J Mol Med 31: 393-399, 2013.
APA
Noh, E., Chung, E.Y., Youn, H.J., Jung, S.H., Hur, H., Lee, Y., & Kim, J. (2013). Cis-guggulsterone inhibits the IKK/NF-κB pathway, whereas trans-guggulsterone inhibits MAPK/AP-1 in MCF‑7 breast cancer cells: Guggulsterone regulates MMP‑9 expression in an isomer-specific manner. International Journal of Molecular Medicine, 31, 393-399. https://doi.org/10.3892/ijmm.2012.1214
MLA
Noh, E., Chung, E. Y., Youn, H. J., Jung, S. H., Hur, H., Lee, Y., Kim, J."Cis-guggulsterone inhibits the IKK/NF-κB pathway, whereas trans-guggulsterone inhibits MAPK/AP-1 in MCF‑7 breast cancer cells: Guggulsterone regulates MMP‑9 expression in an isomer-specific manner". International Journal of Molecular Medicine 31.2 (2013): 393-399.
Chicago
Noh, E., Chung, E. Y., Youn, H. J., Jung, S. H., Hur, H., Lee, Y., Kim, J."Cis-guggulsterone inhibits the IKK/NF-κB pathway, whereas trans-guggulsterone inhibits MAPK/AP-1 in MCF‑7 breast cancer cells: Guggulsterone regulates MMP‑9 expression in an isomer-specific manner". International Journal of Molecular Medicine 31, no. 2 (2013): 393-399. https://doi.org/10.3892/ijmm.2012.1214