The O‑glycosylation mutant osteopontin alters lung cancer cell growth and migration in vitro and in vivo

  • Authors:
    • Arash Minai‑Tehrani
    • Seung‑Hee Chang
    • Seung Bum Park
    • Myung‑Haing Cho
  • View Affiliations

  • Published online on: September 5, 2013     https://doi.org/10.3892/ijmm.2013.1483
  • Pages: 1137-1149
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Abstract

Osteopontin (OPN) is an acidic, glycosylated and phosphorylated protein that plays an essential role in determining the aggressiveness and oncogenic potential of several types of cancer, including lung cancer. The OPN function is highly dependent on post‑translational modification (PTM) and regulation of the processes that involve OPN can be mediated through glycosylation. However, the connection between OPN function and its O‑glycosylation in lung cancer cells has yet to be investigated. In the present study, this issue was addressed by studying the effects of wild‑type (WT) OPN and a triple mutant (TM) of OPN, which was mutated at three O‑glycosylation sites in lung cancer cells. It was shown that OPN WT rather than OPN TM induced the OPN‑mediated signaling pathway. The OPN WT expression enhanced cap‑dependent protein translation, NF‑κB activity and glucose uptake, whereas a reduction was observed in cells treated with OPN TM. The results clearly demonstrated that unlike OPN WT, OPN TM did not increase lung cancer cell growth and migration both in vitro and in a xenograft mouse model. Thus, results of the present study suggested that targeting OPN by introducing OPN TM may be a good strategy for treating lung cancer.
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November 2013
Volume 32 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Minai‑Tehrani A, Chang SH, Park SB and Cho MH: The O‑glycosylation mutant osteopontin alters lung cancer cell growth and migration in vitro and in vivo. Int J Mol Med 32: 1137-1149, 2013.
APA
Minai‑Tehrani, A., Chang, S., Park, S.B., & Cho, M. (2013). The O‑glycosylation mutant osteopontin alters lung cancer cell growth and migration in vitro and in vivo. International Journal of Molecular Medicine, 32, 1137-1149. https://doi.org/10.3892/ijmm.2013.1483
MLA
Minai‑Tehrani, A., Chang, S., Park, S. B., Cho, M."The O‑glycosylation mutant osteopontin alters lung cancer cell growth and migration in vitro and in vivo". International Journal of Molecular Medicine 32.5 (2013): 1137-1149.
Chicago
Minai‑Tehrani, A., Chang, S., Park, S. B., Cho, M."The O‑glycosylation mutant osteopontin alters lung cancer cell growth and migration in vitro and in vivo". International Journal of Molecular Medicine 32, no. 5 (2013): 1137-1149. https://doi.org/10.3892/ijmm.2013.1483