Open Access

Development of a novel liposomal nanodelivery system for bioluminescence imaging and targeted drug delivery in ErbB2-overexpressing metastatic ovarian carcinoma

  • Authors:
    • Xiao-Jian Han
    • Yong-Fang Wei
    • Yu-Ying Wan
    • Li-Ping Jiang
    • Jian-Feng Zhang
    • Hong-Bo Xin
  • View Affiliations

  • Published online on: September 4, 2014     https://doi.org/10.3892/ijmm.2014.1922
  • Pages: 1225-1232
  • Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Liposomes as targeted drug delivery systems are an emerging strategy in the treatment of cancer to selectively target tumors or genes. In this study, we generated the recombinant protein, EC1-GLuc, by fusing the EC1 peptide, an artificial ligand of ErbB2, with Gaussia luciferase (GLuc). The purified EC1-GLuc was conjugated with a nickel-chelating liposome to construct the EC1-GLuc-liposome. In vitro experiments revealed that the EC1-GLuc-liposome selectively targeted and internalized into ErbB2-overexpressing SKOv3 cells for bioluminescence imaging. A cell-impermeable fluorescence dye (HPTS) encapsulated in the EC-GLuc-liposome was efficiently delivered into the SKOv3 cells. In addition, the EC1-GLuc-liposome also targeted metastatic SKOv3 tumors for bioluminescence imaging and effectively delivered HPTS into metastatic tumors in vivo. Therefore, the present study demonstrates the novel EC1-GLuc-liposome to be an effective theranostic system for monitoring and treating ErbB2-overexpressing metastatic ovarian carcinoma through a combination of targeted molecular imaging and DDS.
View Figures
View References

Related Articles

Journal Cover

November-2014
Volume 34 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Han X, Wei Y, Wan Y, Jiang L, Zhang J and Xin H: Development of a novel liposomal nanodelivery system for bioluminescence imaging and targeted drug delivery in ErbB2-overexpressing metastatic ovarian carcinoma. Int J Mol Med 34: 1225-1232, 2014.
APA
Han, X., Wei, Y., Wan, Y., Jiang, L., Zhang, J., & Xin, H. (2014). Development of a novel liposomal nanodelivery system for bioluminescence imaging and targeted drug delivery in ErbB2-overexpressing metastatic ovarian carcinoma. International Journal of Molecular Medicine, 34, 1225-1232. https://doi.org/10.3892/ijmm.2014.1922
MLA
Han, X., Wei, Y., Wan, Y., Jiang, L., Zhang, J., Xin, H."Development of a novel liposomal nanodelivery system for bioluminescence imaging and targeted drug delivery in ErbB2-overexpressing metastatic ovarian carcinoma". International Journal of Molecular Medicine 34.5 (2014): 1225-1232.
Chicago
Han, X., Wei, Y., Wan, Y., Jiang, L., Zhang, J., Xin, H."Development of a novel liposomal nanodelivery system for bioluminescence imaging and targeted drug delivery in ErbB2-overexpressing metastatic ovarian carcinoma". International Journal of Molecular Medicine 34, no. 5 (2014): 1225-1232. https://doi.org/10.3892/ijmm.2014.1922