Rhododendron album Blume inhibits iNOS and COX-2 expression in LPS-stimulated RAW264.7 cells through the downregulation of NF-κB signaling

Park,Ji-Won; Kwon,Ok-Kyoung; Kim,Jung-Hee; Oh,Sei-Ryang; Kim,Jae-Hong; Paik,Jin-Hyub; Marwoto,Bambang; Widjhati,Rifatul; Juniarti,Fifit; Irawan,Doddy; Ahn,Kyung-Seop

doi:10.3892/ijmm.2015.2107

Rhododendron album Blume inhibits iNOS and COX-2 expression in LPS-stimulated RAW264.7 cells through the downregulation of NF-κB signaling

Authors:
- Ji-Won Park
- Ok-Kyoung Kwon
- Jung-Hee Kim
- Sei-Ryang Oh
- Jae-Hong Kim
- Jin-Hyub Paik
- Bambang Marwoto
- Rifatul Widjhati
- Fifit Juniarti
- Doddy Irawan
- Kyung-Seop Ahn
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Affiliations: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk 363-883, Republic of Korea, College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea, International Biological Material Research Center, Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea, Center of Pharmaceutical and Medical Technology, The Agency for the Assessment and Application of Technology (BPPT), Kawasan Puspiptek Serpong, Tangerang, Banten 15314, Indonesia
Published online on: February 25, 2015 https://doi.org/10.3892/ijmm.2015.2107
Pages: 987-994

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Abstract

Rhododendron album Blume (RA) has traditionally been used as an herbal medicine and is considered to have anti-inflammatory properties. In the present study, we screened RA extracts with anti-inflammatory properties. The biological effects of an RA methanol extract (RAME) on inflammation were investigated in lipopolysaccharide (LPS)-stimulated mouse RAW264.7 cells. We investigated the effects of RAME on the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in LPS-stimulated RAW264.7 cells. To explore the anti-inflammatory mechanisms of RAME, we measured the mRNA and protein expression of pro-inflammatory mediators induced by RAME in the LPS-stimulated RAW264.7 cells by RT-PCR and western blot analysis, respectively. RAME significantly inhibited the production of NO, PGE2, interleukin (IL)-6, IL-1β and tumor necrosis factor (TNF)-α in the LPS-stimulated RAW264.7 cells. It also suppressed the mRNA and protein expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) and mitogen-activated protein kinases (MAPKs) with a concomitant decrease in the nuclear translocation of nuclear factor-κB (NF-κB) in the LPS-stimulated RAW264.7 cells. These results indicate that RAME inhibits LPS-induced inflammatory responses. These effects were considered to be strongly associated with the suppression of NF-κB activation. We therefore suggest that RAME may be prove to be an effective therapeutic agent for the treatment of inflammatory diseases.

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