Melia azedarach extract stimulates melanogenesis through increase of tyrosinase-related protein 1 expression in B16F10 mouse melanoma cells

Yao,Cheng; Jin,Cheng  Long; Oh,Inn  Gyung; Park,Chi-Hyun; Chung,Jin  Ho

doi:10.3892/ijmm.2015.2182

Melia azedarach extract stimulates melanogenesis through increase of tyrosinase-related protein 1 expression in B16F10 mouse melanoma cells

Authors:
- Cheng Yao
- Cheng Long Jin
- Inn Gyung Oh
- Chi-Hyun Park
- Jin Ho Chung
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Affiliations: Department of Dermatology, Seoul National University College of Medicine, Seoul 110‑744, Republic of Korea
Published online on: April 14, 2015 https://doi.org/10.3892/ijmm.2015.2182
Pages: 1761-1766

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Abstract

Melia azedarach (MA) has been used in folk medicine in Asia for the treatment of several diseases. Several constituents from MA possess anti-herpetic, anti-angiogenic and anticancer properties. The aim of the present study was to investigate the effect of a 70% ethanol extract of MA on melanogenesis and the underlying mechanisms involved. A B16F10 mouse melanoma cell line was used in our experiments. Treatment of B16F10 cells with the MA extract (10, 20 and 40 µg/ml) increased melanin content in a concentration‑dependent manner without cytotoxicity at 24 h. Further experiments indicated that the MA extract (20 µg/ml) increased melanin content as early as at 4 h after treatment. Additionally, although the MA extract did not affect intracellular tyrosinase activity and the protein levels of tyrosinase and tyrosinase-related protein-2 (TRP-2) at 2 and 4 h after treatment, the MA extract increased TRP-1 protein expression at both time points. However, no significant effect of the MA extract treatment on TRP-1 mRNA level at the time points measured was observed. In conclusion, the results from the present study demonstrate that the MA extract increases melanogenesis through the upregulation of TRP‑1 protein expression by post-transcriptional control in B16F10 cells and suggest that the MA extract can be viewed as a rapid inducer of melanogenesis, thus rendering it a potential treatment for hypopigmentation diseases including vitiligo.

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