Open Access

In-depth analysis of the critical genes and pathways in colorectal cancer

  • Authors:
    • Fuguo Liu
    • Fengzhi Ji
    • Yuling Ji
    • Yueping Jiang
    • Xueguo Sun
    • Yanyan Lu
    • Lingyun Zhang
    • Yue Han
    • Xishuang Liu
  • View Affiliations

  • Published online on: July 30, 2015     https://doi.org/10.3892/ijmm.2015.2298
  • Pages: 923-930
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the molecular targets for colorectal cancer (CRC). Differentially expressed genes (DEGs) were screened between CRC and matched adjacent noncancerous samples. GENETIC_ASSOIATION_DB_DISEASE analysis was performed to identify CRC genes from the identified DEGs using the Database for Annotation, Visualization and Integrated Discovery, followed by Gene Οntology (GO) and Kyoto Encyclopedia of Genes and Genomes analysis for the CRC genes. A protein‑protein interaction (PPI) network was constructed for the CRC genes, followed by determination and analysis of the hub genes, in terms of the protein domains and spatial structure. In total, 35 CRC genes were determined, including 19 upregulated and 16 downregulated genes. Downregulated N‑acetyltransferase (NAT)1 and NAT2 were enriched in the caffeine metabolism pathway. The downregulated and upregulated genes were enriched in a number of GO terms and pathways, respectively. Cyclin D1 (CCND1) and proliferating cell nuclear antigen (PCNA) were identified as the hub genes in the PPI network. The C‑terminal and N‑terminal domains were similar in PCNA, but different in CCND1. The results suggested PCNA, CCND1, NAT1 and NAT2 for use as biomarkers to enable early diagnosis and monitoring of CRC. These results form a basis for developing therapies, which target the unique protein domains of PCNA and CCND1.
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October-2015
Volume 36 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
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Spandidos Publications style
Liu F, Ji F, Ji Y, Jiang Y, Sun X, Lu Y, Zhang L, Han Y and Liu X: In-depth analysis of the critical genes and pathways in colorectal cancer. Int J Mol Med 36: 923-930, 2015.
APA
Liu, F., Ji, F., Ji, Y., Jiang, Y., Sun, X., Lu, Y. ... Liu, X. (2015). In-depth analysis of the critical genes and pathways in colorectal cancer. International Journal of Molecular Medicine, 36, 923-930. https://doi.org/10.3892/ijmm.2015.2298
MLA
Liu, F., Ji, F., Ji, Y., Jiang, Y., Sun, X., Lu, Y., Zhang, L., Han, Y., Liu, X."In-depth analysis of the critical genes and pathways in colorectal cancer". International Journal of Molecular Medicine 36.4 (2015): 923-930.
Chicago
Liu, F., Ji, F., Ji, Y., Jiang, Y., Sun, X., Lu, Y., Zhang, L., Han, Y., Liu, X."In-depth analysis of the critical genes and pathways in colorectal cancer". International Journal of Molecular Medicine 36, no. 4 (2015): 923-930. https://doi.org/10.3892/ijmm.2015.2298