Lysyl oxidase modulates the osteoblast differentiation of primary mouse calvaria cells

  • Authors:
    • Anjali Sharma-Bhandari
    • Sun-Hyang Park
    • Ju-Young Kim
    • Jaemin Oh
    • Youngho Kim
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  • Published online on: October 21, 2015     https://doi.org/10.3892/ijmm.2015.2384
  • Pages: 1664-1670
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Abstract

Lysyl oxidase (LOX) is an extracellular amine oxidase that mediates the formation of collagen fibers. Thus far, five LOX family genes [LOX, lysyl oxidase-like (LOXL)1, LOXL2, LOXL3 and LOXL4] have been identified in humans, each encoding the characteristic C-terminal domains that are required for amine oxidase activity. During osteoblastogenesis, collagen fibers function as a three-dimensional scaffold for organizing mineral deposition. In this study, to assess the functional roles of the LOX family members in osteoblastogenesis, we investigated the temporal expression of these genes as a function of phenotypic development during the osteoblast differentiation of primary cultured mouse calvaria cells. Of the LOX family members, only LOX was prominently expressed during osteoblast differentiation. LOX expression was highest on day 9 of differentiation, as shown by RT-PCR and western blot analysis. The expression pattern of collagen, type I, alpha 2 (COL1A2), which encodes the α2-chain of mouse collagen type I, was similar to that of LOX. The total amine oxidase activity of the differentiating calvaria cells exhibited a temporal pattern that paralleled LOX expression, reaching the highest level on day 9 of differentiation. We also noted that the inhibition of the amine oxidase activity of LOX significantly suppressed both mineral nodule formation and the expression of osteoblast marker genes during the differentiation of primary calvaria cells. Taken together, these findings suggest that the LOX-mediated organization of collagen fibers in the extracellular matrix is an important regulator of osteoblastogenesis.
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December-2015
Volume 36 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Sharma-Bhandari A, Park S, Kim J, Oh J and Kim Y: Lysyl oxidase modulates the osteoblast differentiation of primary mouse calvaria cells. Int J Mol Med 36: 1664-1670, 2015.
APA
Sharma-Bhandari, A., Park, S., Kim, J., Oh, J., & Kim, Y. (2015). Lysyl oxidase modulates the osteoblast differentiation of primary mouse calvaria cells. International Journal of Molecular Medicine, 36, 1664-1670. https://doi.org/10.3892/ijmm.2015.2384
MLA
Sharma-Bhandari, A., Park, S., Kim, J., Oh, J., Kim, Y."Lysyl oxidase modulates the osteoblast differentiation of primary mouse calvaria cells". International Journal of Molecular Medicine 36.6 (2015): 1664-1670.
Chicago
Sharma-Bhandari, A., Park, S., Kim, J., Oh, J., Kim, Y."Lysyl oxidase modulates the osteoblast differentiation of primary mouse calvaria cells". International Journal of Molecular Medicine 36, no. 6 (2015): 1664-1670. https://doi.org/10.3892/ijmm.2015.2384