Functional differences between neonatal and adult fibroblasts and keratinocytes: Donor age affects epithelial-mesenchymal crosstalk in vitro

Mateu,Rosana; Živicová,Veronika; Krejčí,Eliška Drobná; Grim,Miloš; Strnad,Hynek; Vlček,Čestmír; Kolář,Michal; Lacina,Lukáš; Gál,Peter; Borský,Jiří; Smetana Jr,Karel; Dvořánková,Barbora

doi:10.3892/ijmm.2016.2706

Functional differences between neonatal and adult fibroblasts and keratinocytes: Donor age affects epithelial-mesenchymal crosstalk in vitro

Authors:
- Rosana Mateu
- Veronika Živicová
- Eliška Drobná Krejčí
- Miloš Grim
- Hynek Strnad
- Čestmír Vlček
- Michal Kolář
- Lukáš Lacina
- Peter Gál
- Jiří Borský
- Karel Smetana Jr
- Barbora Dvořánková
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Affiliations: Institute of Anatomy, First Faculty of Medicine, Charles University, Prague, Czech Republic, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic vvi, Prague, Czech Republic, Department for Biomedical Research, East-Slovak Institute of Cardiovascular Diseases, Košice, Slovak Republic, Department of ENT, Second Faculty of Medicine, Charles University in Prague and Motol University Hospital, Prague, Czech Republic
Published online on: August 11, 2016 https://doi.org/10.3892/ijmm.2016.2706
Pages: 1063-1074
Copyright: © Mateu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Clinical evidence suggests that healing is faster and almost scarless at an early neonatal age in comparison with that in adults. In this study, the phenotypes of neonatal and adult dermal fibroblasts and keratinocytes (nestin, smooth muscle actin, keratin types 8, 14 and 19, and fibronectin) were compared. Furthermore, functional assays (proliferation, migration, scratch wound closure) including mutual epithelial‑mesenchymal interactions were also performed to complete the series of experiments. Positivity for nestin and α smooth muscle actin was higher in neonatal fibroblasts (NFs) when compared with their adult counterparts (adult fibroblasts; AFs). Although the proliferation of NFs and AFs was similar, they significantly differed in their migration potential. The keratinocyte experiments revealed small, poorly differentiated cells (positive for keratins 8, 14 and 19) in primary cultures isolated from neonatal tissues. Moreover, the neonatal keratinocytes exhibited significantly faster rates of healing the experimentally induced in vitro defects in comparison with adult cells. Notably, the epithelial/mesenchymal interaction studies showed that NFs in co-culture with adult keratinocytes significantly stimulated the adult epithelial cells to acquire the phenotype of small, non-confluent cells expressing markers of poor differentiation. These results indicate the important differences between neonatal and adult cells that may be associated with improved wound healing during the early neonatal period.

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