Role of urotensin II in advanced glycation end product-induced extracellular matrix synthesis in rat proximal tubular epithelial cells

  • Authors:
    • Lin Tian
    • Peng Fu
    • Min Zhou
    • Yunhe Gu
    • Yunqian Li
    • Jiping Qi
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  • Published online on: October 26, 2016     https://doi.org/10.3892/ijmm.2016.2789
  • Pages: 1831-1838
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Abstract

Urotensin II (UII) was first recognized for its constrictive and natriuretic properties in fish almost 40 years ago, and recent studies have suggested that it exerts pro-fibrotic effects in a number of cell lines. In this study, we aimed to evaluate the role of UII in extracellular matrix (ECM) synthesis and secretion in advanced glycation end product (AGE)-stimulated rat proximal tubular epithelial cells (NRK-52E cells). UII promoted the proliferation of the NRK-52E cells in a dose-dependent manner over a concentration range of 10-10-10-8 mol/l and this effect was partly inhibited by both nimodipine and EDTA. Furthermore, AGE-BSA promoted the mRNA and protein expression of UII, fibronectin (FN) and collagen IV (ColIV) in the NRK-52E cells in a dose- and time-dependent manner. In addition, UII promoted the mRNA expression and protein secretion of transforming growth factor (TGF)-β1, FN and Col IV by the NRK-52E cells. Our results suggest that UII promotes the proliferation of NRK-52E cells, an effect which is mediated by the influx of extracellular calcium ions. In addition, our data indicate that AGEs promote UII expression in NRK-52E cells, and that TGF-β1 signaling is a candidate pathway mediating the involvement of UII in renal fibrosis. Collectively, our data suggest that the UII-TGF-β1 signaling may be an important factor in tubulointerstitial nephropathy in diabetes.
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December-2016
Volume 38 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tian L, Fu P, Zhou M, Gu Y, Li Y and Qi J: Role of urotensin II in advanced glycation end product-induced extracellular matrix synthesis in rat proximal tubular epithelial cells. Int J Mol Med 38: 1831-1838, 2016.
APA
Tian, L., Fu, P., Zhou, M., Gu, Y., Li, Y., & Qi, J. (2016). Role of urotensin II in advanced glycation end product-induced extracellular matrix synthesis in rat proximal tubular epithelial cells. International Journal of Molecular Medicine, 38, 1831-1838. https://doi.org/10.3892/ijmm.2016.2789
MLA
Tian, L., Fu, P., Zhou, M., Gu, Y., Li, Y., Qi, J."Role of urotensin II in advanced glycation end product-induced extracellular matrix synthesis in rat proximal tubular epithelial cells". International Journal of Molecular Medicine 38.6 (2016): 1831-1838.
Chicago
Tian, L., Fu, P., Zhou, M., Gu, Y., Li, Y., Qi, J."Role of urotensin II in advanced glycation end product-induced extracellular matrix synthesis in rat proximal tubular epithelial cells". International Journal of Molecular Medicine 38, no. 6 (2016): 1831-1838. https://doi.org/10.3892/ijmm.2016.2789