Open Access

Protective effect of naringin against the LPS-induced apoptosis of PC12 cells: Implications for the treatment of neurodegenerative disorders

  • Authors:
    • Hui Wang
    • You Song Xu
    • Miao Lin Wang
    • Chao Cheng
    • Rui Bian
    • Hao Yuan
    • Yi Wang
    • Ting Guo
    • Lin Lin Zhu
    • Hang Zhou
  • View Affiliations

  • Published online on: February 22, 2017     https://doi.org/10.3892/ijmm.2017.2904
  • Pages: 819-830
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Several studies have demonstrated that increased apoptosis plays an essential role in neurodegenerative disorders. It has been demonstrated that lipopolysaccharide (LPS) induces apoptosis largely through the production of intracellular reactive oxygen species (ROS) and inflammatory mediators. In this study, we investigated the potential protective mechanisms of naringin (Nar), a pummelo peel extract, on LPS-induced PC12 cell apoptosis. Nar pre-conditioning prior to stimulation with LPS for 18 h was a prerequisite for evaluating PC12 cell viability and the protective mechanisms of Nar. Nar significantly improved cell survival in a time- and concentration-dependent manner. On the one hand, Nar downregulated cytochrome P450 2E1 (CYP2E1), inhibited the release of ROS, mitigated the stimulation of oxidative stress, and rectified the antioxidant protein contents of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), superoxide dismutase (SOD)2 and glutathione synthetase (GSS). On the other hand, Nar downregulated inflammatory gene and protein expression, including interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, HMGB1, high mobility group box 1 protein (HMGB1), cyclooxygenase-2 (COX-2), the Toll-like receptor 4 (TLR4)-myeloid differentiation factor 88 (MyD88)-TNF receptor‑associated factor 6 (TRAF6) path­way and downstream mitogen activated protein kinase (MAPK) phosphorylation, activator protein transcription factor-1 (AP-1) and nuclear factor (NF)-κB. Moroever, Nar markedly attenuated the cytochrome c shift from the mitochondria to the cytosol and regulated caspase-3-related protein expression. To the best of our knowledge, this is the first study to report the antioxidant, anti-inflammatory and anti-apoptotic effects of Nar in neuronal-like PC12 cells. These results suggest that Nar can be utilized as a potential drug for the treatment of neurodegenerative disorders.
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April-2017
Volume 39 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wang H, Xu YS, Wang ML, Cheng C, Bian R, Yuan H, Wang Y, Guo T, Zhu LL, Zhou H, Zhou H, et al: Protective effect of naringin against the LPS-induced apoptosis of PC12 cells: Implications for the treatment of neurodegenerative disorders. Int J Mol Med 39: 819-830, 2017
APA
Wang, H., Xu, Y.S., Wang, M.L., Cheng, C., Bian, R., Yuan, H. ... Zhou, H. (2017). Protective effect of naringin against the LPS-induced apoptosis of PC12 cells: Implications for the treatment of neurodegenerative disorders. International Journal of Molecular Medicine, 39, 819-830. https://doi.org/10.3892/ijmm.2017.2904
MLA
Wang, H., Xu, Y. S., Wang, M. L., Cheng, C., Bian, R., Yuan, H., Wang, Y., Guo, T., Zhu, L. L., Zhou, H."Protective effect of naringin against the LPS-induced apoptosis of PC12 cells: Implications for the treatment of neurodegenerative disorders". International Journal of Molecular Medicine 39.4 (2017): 819-830.
Chicago
Wang, H., Xu, Y. S., Wang, M. L., Cheng, C., Bian, R., Yuan, H., Wang, Y., Guo, T., Zhu, L. L., Zhou, H."Protective effect of naringin against the LPS-induced apoptosis of PC12 cells: Implications for the treatment of neurodegenerative disorders". International Journal of Molecular Medicine 39, no. 4 (2017): 819-830. https://doi.org/10.3892/ijmm.2017.2904