Forsythoside A exerts an anti-endotoxin effect by blocking the LPS/TLR4 signaling pathway and inhibiting Tregs in vitro

  • Authors:
    • Xiao-Yan Zeng
    • Wei Yuan
    • Lin Zhou
    • Shi-Xiu Wang
    • Yong Xie
    • Ying-Jun Fu
  • View Affiliations

  • Published online on: May 15, 2017     https://doi.org/10.3892/ijmm.2017.2990
  • Pages: 243-250
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Endotoxins, also referred to as lipopolysaccharides (LPS), are powerful immunostimulators involved in a number of severe diseases. Forsythoside A (FTA), a monomer of phenethyl alcohol glycosides extracted from Forsythia suspensa, has been shown to possess anti-bacterial and immunomodulatory properties. However, it is currently not known whether FTA can counter the adverse effects of endotoxins. We investigated the effect of FTA on LPS-stimulated RAW264.7 cells and primary lymphocytes to determine its molecular mechanism of action. RAW264.7 cells and primary lymphocytes were incubated with or without LPS (100 ng/ml) in the presence or absence of FTA or polymyxin B. We found that FTA increased the viability of LPS-treated RAW264.7 cells and primary lymphocytes suggesting that FTA effectively counters the adverse effects of endotoxins. FTA decreased the percentage of regulatory T cells (Tregs) and inhibited the TLR4/MyD88/NF-κB signaling pathway, downregulating Foxp3, IL-10 and TGF-β1, molecules involved in the immunosuppressive function of Tregs. These findings elucidate the molecular mechanism underlying the anti-endotoxin effects of FTA and suggest its use as a new treatment for LPS-induced diseases.
View Figures
View References

Related Articles

Journal Cover

July-2017
Volume 40 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zeng X, Yuan W, Zhou L, Wang S, Xie Y and Fu Y: Forsythoside A exerts an anti-endotoxin effect by blocking the LPS/TLR4 signaling pathway and inhibiting Tregs in vitro. Int J Mol Med 40: 243-250, 2017.
APA
Zeng, X., Yuan, W., Zhou, L., Wang, S., Xie, Y., & Fu, Y. (2017). Forsythoside A exerts an anti-endotoxin effect by blocking the LPS/TLR4 signaling pathway and inhibiting Tregs in vitro. International Journal of Molecular Medicine, 40, 243-250. https://doi.org/10.3892/ijmm.2017.2990
MLA
Zeng, X., Yuan, W., Zhou, L., Wang, S., Xie, Y., Fu, Y."Forsythoside A exerts an anti-endotoxin effect by blocking the LPS/TLR4 signaling pathway and inhibiting Tregs in vitro". International Journal of Molecular Medicine 40.1 (2017): 243-250.
Chicago
Zeng, X., Yuan, W., Zhou, L., Wang, S., Xie, Y., Fu, Y."Forsythoside A exerts an anti-endotoxin effect by blocking the LPS/TLR4 signaling pathway and inhibiting Tregs in vitro". International Journal of Molecular Medicine 40, no. 1 (2017): 243-250. https://doi.org/10.3892/ijmm.2017.2990