Deoxyactein protects pancreatic β-cells against methylglyoxal-induced oxidative cell damage by the upregulation of mitochondrial biogenesis

  • Authors:
    • Kwang Sik Suh
    • Eun Mi Choi
    • Woon-Won Jung
    • Yu Jin Kim
    • Soo Min Hong
    • So Yong Park
    • Sang Youl Rhee
    • Suk Chon
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  • Published online on: June 12, 2017     https://doi.org/10.3892/ijmm.2017.3018
  • Pages: 539-548
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Abstract

Methylglyoxal (MG) is one of the major precursors of advanced glycation end products (AGEs), which are considered to be one of the causes of diabetes and its complications. The root and rhizomes of black cohosh (Cimicifuga racemosa) have long been used medicinally, and deoxyactein is one of its major constituents. In the present study, the protective effects of deoxyactein against MG-induced oxidative cell damage were investigated in insulin-producing pancreatic β-cells. We found that deoxyactein protected the pancreatic β-cells against MG-induced cell death. Pre-treatment with deoxyactein significantly reduced the levels of intracellular reactive oxygen species (ROS), interleukin-1β (IL-1β), cardiolipin peroxidation, and protein adduct accumulation induced by MG. Pre-treatment of the cells with deoxyactein restored glyoxalase I activity and insulin secretion which were reduced by MG, and increased the mRNA expression of insulin 2 (INS2) and pancreatic and duodenal homeobox protein-1 (PDX-1). It also increased the levels of endogenous antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GPX). Furthermore, treatment with deoxyactein increased the levels of sirtuin 1 (SIRT1) and peroxisome proliferator-activated receptor-γ co-activator-1α (PGC-1α). These findings indicate that deoxyactein may exert beneficial effects on pancreatic β-cells via the upregulation of mitochondrial biogenesis. Taken together, these results suggest that deoxyactein may be used for the prevention of pancreatic β-cell damage.
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August-2017
Volume 40 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Suh KS, Choi EM, Jung W, Kim YJ, Hong SM, Park SY, Rhee SY and Chon S: Deoxyactein protects pancreatic β-cells against methylglyoxal-induced oxidative cell damage by the upregulation of mitochondrial biogenesis. Int J Mol Med 40: 539-548, 2017
APA
Suh, K.S., Choi, E.M., Jung, W., Kim, Y.J., Hong, S.M., Park, S.Y. ... Chon, S. (2017). Deoxyactein protects pancreatic β-cells against methylglyoxal-induced oxidative cell damage by the upregulation of mitochondrial biogenesis. International Journal of Molecular Medicine, 40, 539-548. https://doi.org/10.3892/ijmm.2017.3018
MLA
Suh, K. S., Choi, E. M., Jung, W., Kim, Y. J., Hong, S. M., Park, S. Y., Rhee, S. Y., Chon, S."Deoxyactein protects pancreatic β-cells against methylglyoxal-induced oxidative cell damage by the upregulation of mitochondrial biogenesis". International Journal of Molecular Medicine 40.2 (2017): 539-548.
Chicago
Suh, K. S., Choi, E. M., Jung, W., Kim, Y. J., Hong, S. M., Park, S. Y., Rhee, S. Y., Chon, S."Deoxyactein protects pancreatic β-cells against methylglyoxal-induced oxidative cell damage by the upregulation of mitochondrial biogenesis". International Journal of Molecular Medicine 40, no. 2 (2017): 539-548. https://doi.org/10.3892/ijmm.2017.3018