Protective effect of Rhizoma Dioscoreae extract against alveolar bone loss in ovariectomized rats via regulation of IL-6/STAT3 signaling

  • Authors:
    • Zhi-Guo Zhang
    • Yan-Jing Chen
    • Li-Hua Xiang
    • Jing-Hua Pan
    • Zhen Wang
    • Gary Guishan Xiao
    • Da-Hong Ju
  • View Affiliations

  • Published online on: September 8, 2017     https://doi.org/10.3892/ijmm.2017.3130
  • Pages: 1602-1610
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Abstract

The aim of the present study was to assess the effectiveness of Rhizoma Dioscoreae extract (RDE) on preventing rat alveolar bone loss induced by ovariectomy (OVX), and to determine the role of interleukin-6 (IL-6)/signal transducer and activator of transcription 3 (STAT3) signaling pathway in this effect. Female Wistar rats were subjected to OVX or sham surgery. The rats that had undergone OVX were treated with RDE (RDE group), vehicle (OVX group) or 17β-estradiol subcutaneous injection (E2 group). Subsequently, bone metabolic activity was assessed by analyzing 3-D alveolar bone construction, bone mineral density, as well as the plasma biomarkers of bone turnover. The gene expression of alveolar bone in the OVX and RDE groups was evaluated by IL-6/STAT3 signaling pathway polymerase chain reaction (PCR) arrays, and differentially expressed genes were determined through reverse transcription-quantitative PCR. The inhibitory effect of RDE on alveolar bone loss in the OVX group was demonstrated in the study. In comparison with the OVX group, the RDE group exhibited 19 downregulated genes and 1 upregulated gene associated with the IL-6/STAT3 signaling pathway in alveolar bone. Thus, RDE was shown to relieve OVX-induced alveolar bone loss in rats, an effect which was likely associated with decreased abnormal bone remodeling via regulation of the IL-6/STAT3 signaling pathway.
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November-2017
Volume 40 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhang Z, Chen Y, Xiang L, Pan J, Wang Z, Xiao GG and Ju D: Protective effect of Rhizoma Dioscoreae extract against alveolar bone loss in ovariectomized rats via regulation of IL-6/STAT3 signaling. Int J Mol Med 40: 1602-1610, 2017.
APA
Zhang, Z., Chen, Y., Xiang, L., Pan, J., Wang, Z., Xiao, G.G., & Ju, D. (2017). Protective effect of Rhizoma Dioscoreae extract against alveolar bone loss in ovariectomized rats via regulation of IL-6/STAT3 signaling. International Journal of Molecular Medicine, 40, 1602-1610. https://doi.org/10.3892/ijmm.2017.3130
MLA
Zhang, Z., Chen, Y., Xiang, L., Pan, J., Wang, Z., Xiao, G. G., Ju, D."Protective effect of Rhizoma Dioscoreae extract against alveolar bone loss in ovariectomized rats via regulation of IL-6/STAT3 signaling". International Journal of Molecular Medicine 40.5 (2017): 1602-1610.
Chicago
Zhang, Z., Chen, Y., Xiang, L., Pan, J., Wang, Z., Xiao, G. G., Ju, D."Protective effect of Rhizoma Dioscoreae extract against alveolar bone loss in ovariectomized rats via regulation of IL-6/STAT3 signaling". International Journal of Molecular Medicine 40, no. 5 (2017): 1602-1610. https://doi.org/10.3892/ijmm.2017.3130