RACK1 silencing attenuates renal fibrosis by inhibiting TGF-β signaling

Feng,Jie; Xie,Liyi; Kong,Ranran; Zhang,Yali; Shi,Kehui; Lu,Wanhong; Jiang,Hongli

doi:10.3892/ijmm.2017.3154

RACK1 silencing attenuates renal fibrosis by inhibiting TGF-β signaling

Authors:
- Jie Feng
- Liyi Xie
- Ranran Kong
- Yali Zhang
- Kehui Shi
- Wanhong Lu
- Hongli Jiang
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Affiliations: Department of Nephrology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Thoracic Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Blood Purification Center, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
Published online on: September 27, 2017 https://doi.org/10.3892/ijmm.2017.3154
Pages: 1965-1970

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Abstract

The receptor for activated C-kinase 1 (RACK1) is a member of the WD40-repeat family of proteins and has been reported to be implicated in the development of liver fibrosis. However, the role of RACK1 in renal fibrosis remains unclear. Therefore, in this study, we investigated the effects of RACK1 on transforming growth factor-β1 (TGF-β1)-treated human proximal tubular epithelial cells and aimed to elucidate the possible mechanisms responsible for its anti-fibrotic effects. Our results revealed that RACK1 was highly expressed in the renal fibrotic tissues and TGF-β1-treated HK-2 cells. RACK1 silencing inhibited TGF-β1‑induced α-smooth muscle actin and connective tissue growth factor expression in the HK-2 cells. Furthermore, RACK1 silencing inhibited the expression of phosphorylated Smad3 in the TGF-β1-treated HK-2 cells. To the best of our knowledge, these data demonstrate for the first time the role of RACK1 in renal fibrosis. The present findings indicate that RACK1 silencing attenuates renal fibrosis by suppressing the activation of TGF-β1/Smad3 signaling pathway in HK-2 cells. Thus, RACK1 may serve as a novel regulator of renal fibrosis.

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