Radical scavenging-linked anti-adipogenic activity of Alnus firma extracts

Choi,Sun-Il; Lee,Jong Seok; Lee,Sarah; Lee,Jin-Ha; Yang,Hee-Sun; Yeo,Joohong; Kim,Jong-Yea; Lee,Boo-Yong; Kang,Il-Jun; Lee,Ok-Hwan

doi:10.3892/ijmm.2017.3221

Radical scavenging-linked anti-adipogenic activity of Alnus firma extracts

Authors:
- Sun-Il Choi
- Jong Seok Lee
- Sarah Lee
- Jin-Ha Lee
- Hee-Sun Yang
- Joohong Yeo
- Jong-Yea Kim
- Boo-Yong Lee
- Il-Jun Kang
- Ok-Hwan Lee
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Affiliations: Department of Food Science and Biotechnology, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, National Institute of Biological Resource, Incheon 22689, Republic of Korea, Department of Food Science and Biotechnology, CHA University, Seongnam, Gyeonggi 13488, Republic of Korea, Department of Food Science and Nutrition, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea
Published online on: October 27, 2017 https://doi.org/10.3892/ijmm.2017.3221
Pages: 119-128
Copyright: © Choi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The purpose of the present study was to investigate the antioxidant activity and anti-adipogenic effect of extracts from Alnus firma (A. firma), which is an edible plant that grows in mountainous areas. The total phenolic, flavonoid and anthocyanin content as well as the antioxidant activity of a 70% ethanolic extract of A. firma (AFE) was assessed. Furthermore, the effects of AFE on lipid accumulation and reactive oxygen species (ROS) production during adipogenesis of 3T3-L1 cells were investigated. The results revealed that the total phenolic, flavonoid and pro-anthocyanidin content of AFE as 436.26±3.30 mg gallic acid equivalents/g, 73.82±0.54 mg quercetin equivalents/g and 149.25±6.06 mg catechin equivalents/g, respectively. In addition, AFE exerted significant antioxidant effects in terms of 1,1-diphenyl-2-picryl hydrazyl radical scavenging activity, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity, reducing power, oxygen radical absorbance capacity and nitric oxide radical scavenging activity. As for its anti-adipogenic activity, AFE significantly inhibited ROS production and lipid accumulation during adipogenesis of 3T3-L1 cells compared with those in control cells. In addition, AFE regulated adipogenic transcription factors including peroxisome proliferator‑activated receptor-γ, CCAAT/enhance-binding protein α and adipocyte protein 2. These results indicated that A. firma is a potential candidate for a functional food supplement.

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