Anti‑inflammatory effect of quercetin and galangin in LPS‑stimulated RAW264.7 macrophages and DNCB‑induced atopic dermatitis animal models

Lee,Hae Nim; Shin,Seong Ah; Choo,Gang Sik; Kim,Hyeong Jin; Park,Young Seok; Kim,Byeong Soo; Kim,Sang Ki; Cho,Sung Dae; Nam,Jeong Seok; Choi,Chang Sun; Che,Jeong Hwan; Park,Byung Kwon; Jung,Ji Youn

doi:10.3892/ijmm.2017.3296

Anti‑inflammatory effect of quercetin and galangin in LPS‑stimulated RAW264.7 macrophages and DNCB‑induced atopic dermatitis animal models

Authors:
- Hae Nim Lee
- Seong Ah Shin
- Gang Sik Choo
- Hyeong Jin Kim
- Young Seok Park
- Byeong Soo Kim
- Sang Ki Kim
- Sung Dae Cho
- Jeong Seok Nam
- Chang Sun Choi
- Jeong Hwan Che
- Byung Kwon Park
- Ji Youn Jung
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Affiliations: Department of Companion and Laboratory Animal Science, Kongju National University, Yesan 340702, Republic of Korea, Department of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul 03080, Republic of Korea, Gwangju Institute of Science and Technology, School of Life Sciences, Gwangju 500712, Republic of Korea, School of Food Science and Technology, Chung‑Ang University, Ansung 456756, Republic of Korea, Biomedical Center for Animal Resource Development, Seoul National University College of Medicine, Seoul 03080, Republic of Korea
Published online on: November 29, 2017 https://doi.org/10.3892/ijmm.2017.3296
Pages: 888-898
Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Flavonols are compounds that have been shown to possess potent anti‑inflammatory effects in cellular and animal models of inflammation. In the present study, the anti‑inflammatory effects and mechanisms of two natural flavonols, quercetin and galangin, in lipopolysaccharide (LPS)‑stimulated RAW264.7 macrophages were investigated. It was identified that quercetin and galangin markedly reduced the production of nitric oxide (NO), inducible NO synthase and interleukin‑6, and the nuclear translocation of nuclear factor‑κB (NF‑κB). In addition, LPS‑induced activation of extracellular signal‑regulated kinase 1/2 (Erk1/2) and c‑Jun N‑terminal kinase (JNK) was suppressed by quercetin and galangin. Taken together, these data implied that NF‑κB, Erk1/2 and JNK may be potential molecular targets of quercetin and galangin in an LPS‑induced inflammatory response. Subsequently, the effects of oral administration of quercetin or galangin, either alone or in combination, in a 2,4‑dinitrochlorobenzene‑induced atopic dermatitis (AD) mouse model were investigated. As a result, measurements of ear thickness and the levels of serum immunoglobulin E, and histological analysis revealed that the two flavonols led to a decrease in inflammation, whereas, in combination, they were even more effective. These results suggested that quercetin and galangin may be promising therapeutic agents for AD. Additionally, their combination may be a novel therapeutic strategy for the prevention of AD.

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