Effect of phlorotannins on myofibroblast differentiation and ECM protein expression in transforming growth factor β1‑induced nasal polyp‑derived fibroblasts

Park,Jungik; Choi,Grace; Yim,Mi‑Jin; Lee,Jeong Min; Yoo,Jong Su; Park,Won Sun; Park,Seong Kook; Park,Saegwang; Seo,Su‑Kil; Kim, Tae‑Geum; Lee,Dae‑Sung; Choi,Il‑Whan

doi:10.3892/ijmm.2018.3770

Effect of phlorotannins on myofibroblast differentiation and ECM protein expression in transforming growth factor β1‑induced nasal polyp‑derived fibroblasts

Authors:
- Jungik Park
- Grace Choi
- Mi‑Jin Yim
- Jeong Min Lee
- Jong Su Yoo
- Won Sun Park
- Seong Kook Park
- Saegwang Park
- Su‑Kil Seo
- Tae‑Geum Kim
- Dae‑Sung Lee
- Il‑Whan Choi
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Affiliations: Division of Endocrinology and Metabolism, Department of Internal Medicine, Dong‑Eui Medical Center, Dong‑Eui University, Busan 47227, Republic of Korea, Department of Applied Research, National Marine Biodiversity Institute of Korea, Seocheon, Chungcheongnam‑do 33662, Republic of Korea, Department of Physiology, Kangwon National University School of Medicine, Chuncheon, Gangwon‑do 24341, Republic of Korea , Department of Otorhinolaryngology Head and Neck Surgery, Busan Paik Hospital, Inje University, Busan 47392, Republic of Korea , Department of Microbiology and Immunology, College of Medicine, Inje University, Busan 47392, Republic of Korea , Center for Jeongup Industry‑Academy‑Institute Cooperation, Chonbuk National University, Jeonju, Jeollabuk‑do 54896, Republic of Korea
Published online on: July 12, 2018 https://doi.org/10.3892/ijmm.2018.3770
Pages: 2213-2220

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Abstract

Phlorotannins (PTNs), a group of phenolic compounds from seaweeds, have diverse bioactivities. However, there has been no report on their antifibrotic effects during nasal polyp (NP) formation. In the present study, the effect of PTNs on transforming growth factor (TGF)‑β1‑induced profibrotic responses in nasal polyp‑derived fibroblasts (NPDFs) were determined and the relevant signaling pathways were investigated. The expression levels of collagen type‑1 (Col‑1) and fibronectin in NP tissues were measured by western blot analysis and immunohistochemistry. The NPDFs were treated with TGF‑β1 (1 ng/ml) in the presence or absence of PTNs (5‑30 µg/ml). The expression levels of α‑smooth muscle actin (α‑SMA), Col‑1, fibronectin, and phosphorylated‑small mothers against decapentaplegic (Smad)2/3 in NPDFs were measured by western blot analysis. The contractile activity of the NPDFs was determined by a collagen gel contraction assay. Col‑1 and fibronectin proteins were found to be expressed in NP tissues. PTNs had no significant cytotoxic effect on TGF‑β1‑induced NPDFs. TGF‑β1 induced the expression α‑SMA, Col‑1 and fibronectin, and stimulated fibroblast‑mediated contraction of collagen gel. However, pre‑treatment with PTNs inhibited the expression of these proteins. The inhibitory effects were mediated through the suppression of Smad2/3 signaling pathways in TGF‑β1‑induced NPDFs. These resulted suggested that PTNs may be important in inhibiting myofibroblast differentiation and extracellular matrix protein accumulation in NP formation through the Smad2/3 signaling pathway.

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