Open Access

Pterostilbene inhibits nutrient metabolism and induces apoptosis through AMPK activation in multiple myeloma cells

  • Authors:
    • Huiling Mei
    • Yu Xiang
    • Heng Mei
    • Bin Fang
    • Qiuguo Wang
    • Dedong Cao
    • Yu Hu
    • Tao Guo
  • View Affiliations

  • Published online on: September 5, 2018     https://doi.org/10.3892/ijmm.2018.3857
  • Pages: 2676-2688
  • Copyright: © Mei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Multiple myeloma (MM) cells are characterized by an abnormal nutrient metabolism that is distinct from normal plasma cells. Pterostilbene (PTE), a bioactive component of blueberries, has been demonstrated to induce apoptosis in multiple types of cancer cell. The present study evaluated whether PTE treatment affected the survival of MM cells from a metabolic perspective, and the potential mechanisms of this. It was observed that the administration of PTE induced apoptosis, which was mediated by the increased activation of AMP‑activated protein kinase (AMPK). Once activated, AMPK decreased the expression and/or activity of key lipogenic enzymes, including fatty acid synthase and acetyl‑CoA carboxylase. In addition, the activation of AMPK suppressed the downstream substrate, mechanistic target of rapamycin, which dephosphorylated eukaryotic initiation factor 4E‑binding protein 1, leading to a general decrease in mRNA translation. Pre‑treatment with the AMPK inhibitor compound C prior to PTE treatment compromised the anti‑myeloma apoptosis effect, suggesting the critical role of AMPK in mediating PTE‑induced cell toxicity. Consistent results were obtained in vivo. Finally, autophagy was adaptively upregulated subsequent to PTE treatment; the pro‑apoptotic efficacy of PTE was potentiated once autophagic flux was inhibited by 3‑methyladenine. Taken together, these data demonstrated that PTE exerts anti‑tumor effects on MM cells via AMPK‑induced nutrient suppression.
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November-2018
Volume 42 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Mei H, Xiang Y, Mei H, Fang B, Wang Q, Cao D, Hu Y and Guo T: Pterostilbene inhibits nutrient metabolism and induces apoptosis through AMPK activation in multiple myeloma cells. Int J Mol Med 42: 2676-2688, 2018.
APA
Mei, H., Xiang, Y., Mei, H., Fang, B., Wang, Q., Cao, D. ... Guo, T. (2018). Pterostilbene inhibits nutrient metabolism and induces apoptosis through AMPK activation in multiple myeloma cells. International Journal of Molecular Medicine, 42, 2676-2688. https://doi.org/10.3892/ijmm.2018.3857
MLA
Mei, H., Xiang, Y., Mei, H., Fang, B., Wang, Q., Cao, D., Hu, Y., Guo, T."Pterostilbene inhibits nutrient metabolism and induces apoptosis through AMPK activation in multiple myeloma cells". International Journal of Molecular Medicine 42.5 (2018): 2676-2688.
Chicago
Mei, H., Xiang, Y., Mei, H., Fang, B., Wang, Q., Cao, D., Hu, Y., Guo, T."Pterostilbene inhibits nutrient metabolism and induces apoptosis through AMPK activation in multiple myeloma cells". International Journal of Molecular Medicine 42, no. 5 (2018): 2676-2688. https://doi.org/10.3892/ijmm.2018.3857