Open Access

NLRP3 inflammasome and lipid metabolism analysis based on UPLC-Q-TOF-MS in gouty nephropathy

  • Authors:
    • Yan‑Zi Zhang
    • Xiao‑Lu Sui
    • Yun‑Peng Xu
    • Feng‑Juan Gu
    • Ai‑Sha Zhang
    • Ji‑Hong Chen
  • View Affiliations

  • Published online on: April 30, 2019     https://doi.org/10.3892/ijmm.2019.4176
  • Pages: 172-184
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

To determine the differences in plasma metabolism between healthy patients and patients with hyperuricaemia and gouty nephropathy, the present study identified differentially expressed metabolites associated with gouty nephropathy. Furthermore, the NLRP3 inflammasome signalling pathway in gouty nephropathy was explored, and the mechanism of hyperuricaemia‑induced renal damage. Adult male patients examined between July 2016 and June 2017 were selected as the patient cohort for the present study from the Affiliated Bao'an Hospital of Shenzhen, Southern Medical University (Shenzhen, China). These patients were divided into three groups of 30 patients each: Control, hyperuricaemia and gouty nephropathy groups. The expression levels of NLRP3, ASC and caspase‑1 mRNA and protein were detected in peripheral blood mononuclear cells, and the plasma levels of IL‑1β and IL‑18. Ultra‑performance liquid chromatography coupled with quadrupole time‑of‑flight mass spectrometry was used to determine differential levels of metabolites between patients from different groups, in order to identify potential biomarkers. The expression of the NLRP3 inflammasome in peripheral blood mononuclear cells, and the levels of IL‑1β and IL‑18 in the plasma were increased in the gouty nephropathy group compared with the control and hyperuricaemia groups. In addition, 46 metabolites were identified as potential plasma metabolic biomarkers that were able to distinguish gouty nephropathy from hyperuricaemia. The majority of these metabolites were involved in lipid metabolism, in particular the activity of phospholipase Α2 and β‑oxidation. These data indicated that lipid metabolism and the NLRP3 inflammasome serve a pivotal role in gouty nephropathy. In addition, the results suggested that lipids may mediate the progression of gouty nephropathy through the activity of phospholipase A2, β‑oxidation and activation of the NLRP3 inflammasome.
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July-2019
Volume 44 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhang YZ, Sui XL, Xu YP, Gu FJ, Zhang AS and Chen JH: NLRP3 inflammasome and lipid metabolism analysis based on UPLC-Q-TOF-MS in gouty nephropathy. Int J Mol Med 44: 172-184, 2019.
APA
Zhang, Y., Sui, X., Xu, Y., Gu, F., Zhang, A., & Chen, J. (2019). NLRP3 inflammasome and lipid metabolism analysis based on UPLC-Q-TOF-MS in gouty nephropathy. International Journal of Molecular Medicine, 44, 172-184. https://doi.org/10.3892/ijmm.2019.4176
MLA
Zhang, Y., Sui, X., Xu, Y., Gu, F., Zhang, A., Chen, J."NLRP3 inflammasome and lipid metabolism analysis based on UPLC-Q-TOF-MS in gouty nephropathy". International Journal of Molecular Medicine 44.1 (2019): 172-184.
Chicago
Zhang, Y., Sui, X., Xu, Y., Gu, F., Zhang, A., Chen, J."NLRP3 inflammasome and lipid metabolism analysis based on UPLC-Q-TOF-MS in gouty nephropathy". International Journal of Molecular Medicine 44, no. 1 (2019): 172-184. https://doi.org/10.3892/ijmm.2019.4176