Open Access

Screening and identification of biomarkers for systemic sclerosis via microarray technology

  • Authors:
    • Chen Xu
    • Ling‑Bing Meng
    • Yu‑Chen Duan
    • Yong‑Jing Cheng
    • Chun‑Mei Zhang
    • Xing Zhou
    • Ci‑Bo Huang
  • View Affiliations

  • Published online on: September 5, 2019     https://doi.org/10.3892/ijmm.2019.4332
  • Pages: 1753-1770
  • Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Systemic sclerosis (SSc) is a complex autoimmune disease. The pathogenesis of SSc is currently unclear, although like other rheumatic diseases its pathogenesis is complicated. However, the ongoing development of bioinformatics technology has enabled new approaches to research this disease using microarray technology to screen and identify differentially expressed genes (DEGs) in the skin of patients with SSc compared with individuals with healthy skin. Publicly available data were downloaded from the Gene Expression Omnibus (GEO) database and intra‑group data repeatability tests were conducted using Pearson’s correlation test and principal component analysis. DEGs were identified using an online tool, GEO2R. Functional annotation of DEGs was performed using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Finally, the construction and analysis of the protein‑protein interaction (PPI) network and identification and analysis of hub genes was carried out. A total of 106 DEGs were detected by the screening of SSc and healthy skin samples. A total of 10 genes [interleukin‑6, bone morphogenetic protein 4, calumenin (CALU), clusterin, cysteine rich angiogenic inducer 61, serine protease 23, secretogranin II, suppressor of cytokine signaling 3, Toll‑like receptor 4 (TLR4), tenascin C] were identified as hub genes with degrees ≥10, and which could sensitively and specifically predict SSc based on receiver operator characteristic curve analysis. GO and KEGG analysis showed that variations in hub genes were mainly enriched in positive regulation of nitric oxide biosynthetic processes, negative regulation of apoptotic processes, extracellular regions, extracellular spaces, cytokine activity, chemo‑attractant activity, and the phosphoinositide 3 kinase‑protein kinase B signaling pathway. In summary, bioinformatics techniques proved useful for the screening and identification of biomarkers of disease. A total of 106 DEGs and 10 hub genes were linked to SSc, in particular the TLR4 and CALU genes.
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November-2019
Volume 44 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
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Spandidos Publications style
Xu C, Meng LB, Duan YC, Cheng YJ, Zhang CM, Zhou X and Huang CB: Screening and identification of biomarkers for systemic sclerosis via microarray technology. Int J Mol Med 44: 1753-1770, 2019.
APA
Xu, C., Meng, L., Duan, Y., Cheng, Y., Zhang, C., Zhou, X., & Huang, C. (2019). Screening and identification of biomarkers for systemic sclerosis via microarray technology. International Journal of Molecular Medicine, 44, 1753-1770. https://doi.org/10.3892/ijmm.2019.4332
MLA
Xu, C., Meng, L., Duan, Y., Cheng, Y., Zhang, C., Zhou, X., Huang, C."Screening and identification of biomarkers for systemic sclerosis via microarray technology". International Journal of Molecular Medicine 44.5 (2019): 1753-1770.
Chicago
Xu, C., Meng, L., Duan, Y., Cheng, Y., Zhang, C., Zhou, X., Huang, C."Screening and identification of biomarkers for systemic sclerosis via microarray technology". International Journal of Molecular Medicine 44, no. 5 (2019): 1753-1770. https://doi.org/10.3892/ijmm.2019.4332