Open Access

MicroRNA‑449a regulates the progression of brain aging by targeting SCN2B in SAMP8 mice

  • Authors:
    • Ya‑Xin Tan
    • Ying Hong
    • Shui Jiang
    • Min‑Nan Lu
    • Shan Li
    • Bo Chen
    • Li Zhang
    • Tao Hu
    • Rui Mao
    • Rong Mei
    • Yan‑Bin Xiyang
  • View Affiliations

  • Published online on: February 13, 2020     https://doi.org/10.3892/ijmm.2020.4502
  • Pages: 1091-1102
  • Copyright: © Tan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Our previous study demonstrated that the expression of sodium channel voltage‑gated beta 2 (SCN2B) increased with aging in senescence‑accelerated mouse prone 8 (SAMP8) mice, and was identified to be associated with a decline in learning and memory, while the underlying mechanism is unclear. In the present study, multiple differentially expressed miRNAs, which may be involved in the process of aging by regulating target genes, were identified in the prefrontal cortex and hippocampus of SAMP8 mice though miRNA microarray analysis. Using bioinformatics prediction, SCN2B was identified to be one of the potential target genes of miR‑449a, which was downregulated in the hippocampus. Previous studies demonstrated that miR‑449a is involved in the occurrence and progression of aging by regulating a variety of target genes. Therefore, it was hypothesized that miR‑449a may be involved in the process of brain aging by targeting SCN2B. To verify this hypothesis, the following experiments were conducted: A reverse transcription‑quantitative polymerase chain reaction assay revealed that the expression level of miR‑449a was significantly decreased in the prefrontal cortex and hippocampus of 12‑month old SAMP8 mice; a dual‑luciferase reporter assay verified that miR‑449a regulated SCN2B expression by binding to the 3'‑UTR ‘seed region’; an anti‑Ago co‑immunoprecipitation combined with Affymetrix microarray analyses demonstrated that the target mRNA highly enriched with Ago‑miRNPs was confirmed to be SCN2B. Finally, overexpression of miR‑449a or inhibition of SCN2B promoted the extension of hippocampal neurons in vitro. The results of the present study suggested that miR‑449a was downregulated in the prefrontal cortex and hippocampus of SAMP8 mice and may regulate the process of brain aging by targeting SCN2B.
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April-2020
Volume 45 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
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Spandidos Publications style
Tan YX, Hong Y, Jiang S, Lu MN, Li S, Chen B, Zhang L, Hu T, Mao R, Mei R, Mei R, et al: MicroRNA‑449a regulates the progression of brain aging by targeting SCN2B in SAMP8 mice. Int J Mol Med 45: 1091-1102, 2020.
APA
Tan, Y., Hong, Y., Jiang, S., Lu, M., Li, S., Chen, B. ... Xiyang, Y. (2020). MicroRNA‑449a regulates the progression of brain aging by targeting SCN2B in SAMP8 mice. International Journal of Molecular Medicine, 45, 1091-1102. https://doi.org/10.3892/ijmm.2020.4502
MLA
Tan, Y., Hong, Y., Jiang, S., Lu, M., Li, S., Chen, B., Zhang, L., Hu, T., Mao, R., Mei, R., Xiyang, Y."MicroRNA‑449a regulates the progression of brain aging by targeting SCN2B in SAMP8 mice". International Journal of Molecular Medicine 45.4 (2020): 1091-1102.
Chicago
Tan, Y., Hong, Y., Jiang, S., Lu, M., Li, S., Chen, B., Zhang, L., Hu, T., Mao, R., Mei, R., Xiyang, Y."MicroRNA‑449a regulates the progression of brain aging by targeting SCN2B in SAMP8 mice". International Journal of Molecular Medicine 45, no. 4 (2020): 1091-1102. https://doi.org/10.3892/ijmm.2020.4502