Tat‑aldose reductase prevents dopaminergic neuronal cell death by inhibiting oxidative stress and MAPK activation

Cho,Su Bin; Eum,Won Sik; Shin,Min Jea; Yeo,Hyeon Ji; Yeo,Eun Ji; Choi,Yeon Joo; Kwon,Hyun Jung; Cho,Sung‑Woo; Park,Jinseu; Han,Kyu Hyung; Lee,Keun Wook; Park,Jong Kook; Kim,Duk‑Soo; Kim,Dae Won; Choi,Soo Young

doi:10.3892/ijmm.2020.4812

Tat‑aldose reductase prevents dopaminergic neuronal cell death by inhibiting oxidative stress and MAPK activation

Authors:
- Su Bin Cho
- Won Sik Eum
- Min Jea Shin
- Hyeon Ji Yeo
- Eun Ji Yeo
- Yeon Joo Choi
- Hyun Jung Kwon
- Sung‑Woo Cho
- Jinseu Park
- Kyu Hyung Han
- Keun Wook Lee
- Jong Kook Park
- Duk‑Soo Kim
- Dae Won Kim
- Soo Young Choi
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Affiliations: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea, Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangneung‑Wonju National University, Gangneung, Gangwon 25457, Republic of Korea, Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea, Department of Anatomy and BK21 Plus Center, College of Medicine, Soonchunhyang University, Cheonan‑si, Chungcheong 31538, Republic of Korea
Published online on: December 8, 2020 https://doi.org/10.3892/ijmm.2020.4812
Pages: 751-760

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Abstract

Aldose reductase (AR) is known to detoxify aldehydes and prevent oxidative stress. Although AR exerts antioxidant effects, the role of AR in Parkinson's disease (PD) remains unclear. The objective of the present study was to investigate the protective effects of AR protein against 1‑methyl‑4‑phenylpyridinium (MPP+)‑induced SH‑SY5Y cell death and 1‑methyl‑4‑phenyl‑1,2,3,6‑tetrahydropyridine (MPTP)‑induced PD in a mouse model using the cell permeable Tat‑AR fusion protein. The results revealed that when Tat‑AR protein was transduced into SH‑SY5Y cells, it markedly protected the cells against MPP+‑induced death and DNA fragmentation. It also reduced the activation of mitogen-activated protein kinase (MAPKs) and regulated the expression levels of Bcl‑2, Bax and caspase‑3. Immunohistochemical analysis revealed that when Tat‑AR protein was transduced into the substantia nigra (SN) of mice with PD, it markedly inhibited dopaminergic neuronal cell death. Therefore, Tat‑AR may be useful as a therapeutic protein for PD.

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