Interleukin‑27 ameliorates allergic asthma by alleviating the lung Th2 inflammatory environment

Lu,Jiameng; Ji,Xiaoqing; Wang,Lixia; Sun,Fei; Huang,Chuanjun; Peng,Haiying; Jiang,Yunxiu; Guo,Zihan; Liu,Xinyi; Ji,Yanbo; Lu,Degan

doi:10.3892/ijmm.2022.5142

Interleukin‑27 ameliorates allergic asthma by alleviating the lung Th2 inflammatory environment

Authors:
- Jiameng Lu
- Xiaoqing Ji
- Lixia Wang
- Fei Sun
- Chuanjun Huang
- Haiying Peng
- Yunxiu Jiang
- Zihan Guo
- Xinyi Liu
- Yanbo Ji
- Degan Lu
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Affiliations: School of Microelectronics, Shandong University, Jinan, Shandong 250100, P.R. China, Department of Nursing, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan, Shandong 250014, P.R. China, Division of Disinfectant and Supply, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China, Graduate School of Shandong First Medical University, Jinan, Shandong 250000, P.R. China, Department of Respiratory Medicine, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Shandong Institute of Respiratory Diseases, Shandong Institute of Anesthesia and Respiratory Critical Medicine, Jinan, Shandong 250014, P.R. China, Graduate School of Shandong First Medical University, Jinan, Shandong 250000, P.R. China
Published online on: May 5, 2022 https://doi.org/10.3892/ijmm.2022.5142
Article Number: 86
Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Interleukin (IL)‑27 can inhibit the differentiation of Th2 cells and plays a role in the development of asthma. However, whether the therapeutic administration of IL‑27 in a mouse model of asthma can inhibit allergic responses remains a matter of debate. Additionally, the mechanisms through which IL‑27 ameliorates inflammatory responses in asthma are not yet fully understood. Thus, the aim of the present study was to examine the effects of IL‑27 on asthma using a mouse model and to elucidate the underlying mechanisms. For this purpose, mice received an intranasal administration of IL‑27 and the total and differential cell counts, levels of cytokines and type 1 regulatory T (Tr1) cells in the lungs were detected. The protein and mRNA levels of signal transducer and activator of transcription (STAT)1 and STAT3 were analyzed and airway remodeling was assessed. The results indicated that IL‑27 did not ameliorate airway inflammation, airway hyperresponsiveness, and airway remolding when administrated therapeutically. Preventatively, the administration of IL‑27 decreased the concentrations of Th2 cytokines and increased the number of Tr1 cells. The protein and mRNA levels of STAT1 and STAT3 were increased. Taken together, these findings demonstrate that the prophylactic administration of IL‑27 ameliorates asthma by alleviating the lung Th2 inflammatory environment through the restoration of both the STAT1 and STAT3 pathways. IL‑27 may thus prove to be useful as a novel agent for the prevention of asthma.

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